Evidence map›Paper›PMID 33654897›Full record

ArticleBio-protocol2019

Immunofluorescence-based Determination of Centrosome Number in Tissue Samples.

Mengdie Wang, Gregory C Rogers, Anne E Cress

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Mengdie WangCancer Biology Research Program/University of Arizona Cancer Center, Tucson, AZ, USA.
Gregory C RogersCancer Biology Research Program/University of Arizona Cancer Center, Tucson, AZ, USA.
Anne E CressCancer Biology Research Program/University of Arizona Cancer Center, Tucson, AZ, USA.

Funding

VITAMIN AP30CA023074 · NCI · UNIVERSITY OF ARIZONA · PI Teri L Bankhead · 1985 to 2026
$110.2M
Human Prostate Cancer Metastasis and Laminin Binding IntegrinsR01CA159406 · NCI · UNIVERSITY OF ARIZONA · PI CRESS, ANNE E · 2011 to 2015
$1.5M
Identifying molecular mechanisms that suppress centriole amplification.R01GM110166 · NIGMS · UNIVERSITY OF ARIZONA · PI ROGERS, GREGORY CHARLES · 2015 to 2019
$1.4M
Inherent mechanisms that govern centrosome function and duplicationR01GM126035 · NIGMS · UNIVERSITY OF ARIZONA · PI ROGERS, GREGORY CHARLES · 2018 to 2020
$1.0M
NCI NIH HHS P30 CA023074NCI NIH HHS R01 CA159406NIGMS NIH HHS R01 GM110166NIGMS NIH HHS R01 GM126035
6 · The paper itself

Abstract

Centrosome numerical abnormalities have been reported in a variety of tumors. Centrosome numbers in cancer cells display both inter-tumor and intra-tumor heterogeneity. The over production of centrosomes (centrosome amplification) is unique in cancer cells and is a promising target for therapy. Thus, a method to quantify centrosome numbers on a single cell level is needed. Here, we describe a protocol to quantify centrosome numbers in formalin fixed paraffin embedded (FFPE) tissue samples by multiplexing antibodies to define

Indexed as

CancerCentrosomeFFPEHigh resolution immunofluorescent microscopyTissue

Identifiers

PMID33654897
PMCPMC7854016

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.