ArticleBio-protocol2019
Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation.
Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Extraction of high-molecular-weight DNA from Streptococcus spp. for nanopore sequencing in resource-limited settings.MicrobiologyOpen · 2024Article
- Silica-coated magnetic particles for efficient RNA extraction for SARS-CoV-2 detection.Heliyon · 2024Article
- Quantification of Circular RNAs Using Digital Droplet PCR.Journal of visualized experiments : JoVE · 2022Article
- Polydopamine-Coated Magnetic Iron Oxide Nanoparticles: From Design to Applications.Nanomaterials (Basel, Switzerland) · 2022Review
- SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP.Viruses · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purification of nucleic acids is one of the most common procedures employed in modern molecular biology laboratories. Typically, commercial column-based protocols are utilized to isolate DNA or RNA from various sources. However, these methods not only require specialized equipment, but are also extremely expensive for high-throughput applications. Although an elegant answer to this issue can be provided by paramagnetic beads, bead-based open-source protocols have been limited in the past. Here, we provide an easy to follow step-by-step manual for the synthesis of paramagnetic beads, as well as their functionalization with either a silica- or a carboxyl-surface that can be used to replace the commercial columns with self-made magnetic beads. Together with a variety of detailed protocols for their use in high-throughput nucleic acids extractions, this bead synthesis method forms the recently published open platform Bio-On-Magnetic-Beads (BOMB), which is available on PLOS Biology ( Oberacker
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Registered trials
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