ArticleBio-protocol2019
QUEEN-based Spatiotemporal ATP Imaging in Budding and Fission Yeast.
Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments.Biotechnology and bioengineering · 2025Article
- Current and novel approaches in yeast cell death research.Cell death and differentiation · 2025Review
- Polysome collapse and RNA condensation fluidize the cytoplasm.Molecular cell · 2024Article
- Four ways of implementing robustness quantification in strain characterisation.Biotechnology for biofuels and bioproducts · 2023Article
- Article
- Confocal imaging of biomarkers at a single-cell resolution: quantifying 'living' in 3D-printable engineered living material based on Pluronic F-127 and yeast Saccharomyces cerevisiae.Biomaterials research · 2022Article
- Article
- Assessment of Cytotoxicity of α-Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Dual Nanosensor Approach to Determine the Cytosolic Concentration of ATP in Astrocytes.Frontiers in cellular neuroscience · 2020Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeasts have provided an exceptional model for studying metabolism and bioenergetics in eukaryotic cells. Among numerous metabolites, adenosine triphosphate (ATP) is a major metabolite that is essential for all living organisms. Therefore, a clearer understanding of ATP dynamics in living yeast cells is important for deciphering cellular energy metabolism. However, none of the methods currently available to measure ATP, including biochemical analyses and ATP indicators, have been suitable for close examinations of ATP concentrations in yeast cells at the single cell level. Using the recently developed ATP biosensor QUEEN, which is suitable for yeasts and bacteria, a protocol was described herein to visualize ATP concentrations in living budding and fission yeast cells. This simple method enables the easy and reliable examination of ATP dynamics in various yeast mutants, thereby providing novel molecular insights into cellular energy metabolism.
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Registered trials
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