Evidence map›Paper›PMID 33654751›Full record

ArticleBio-protocol2020

Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry.

Claudia Cattoglio, Xavier Darzacq, Robert Tjian, Anders S Hansen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Claudia CattoglioDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Xavier DarzacqDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Robert TjianDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Anders S HansenDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.

Funding

Center for 3D Structure and Physics of the GenomeU54DK107980 · NIDDK · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI DEKKER, JOB, MIRNY, LEONID A · 2015 to 2019
$14.9M
Tools For Imaging the Functional Genome in Living Cells and TissuesU01EB021236 · NIBIB · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI DARZACQ, XAVIER, SINGER, ROBERT H · 2015 to 2017
$2.5M
Molecular mechanisms regulating chromatin looping in time and spaceK99GM130896 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HANSEN, ANDERS SEJR · 2019 to 2019
$94k
NIBIB NIH HHS U01 EB021236NIDDK NIH HHS U54 DK107980NIGMS NIH HHS K99 GM130896
6 · The paper itself

Abstract

Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or require sophisticated experimental and computational infrastructure (

Indexed as

Absolute quantificationFlow cytometryHalo labelingHaloTagNumber of proteins per cellProtein abundanceStandard cell line

Identifiers

PMID33654751
PMCPMC7842713

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.