Evidence map›Paper›PMID 33654738›Full record

ArticleBio-protocol2020

Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in

Nancy Meyer, Omar Davulcu, Qing Xie, Mark Silveria, Grant M Zane, Edward Large, Michael S Chapman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Cryo-EM structure of adeno-associated virus 4 at 2.2 Å resolution.Acta crystallographica. Section D, Structural biology · 2023
    Article
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nancy MeyerDepartment of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, United States.
Omar DavulcuDepartment of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, United States.
Qing XieDepartment of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, United States.
Mark SilveriaDepartment of Biochemistry, University of Missouri, Columbia, United States.
Grant M ZaneDepartment of Biochemistry, University of Missouri, Columbia, United States.
Edward LargeDepartment of Biochemistry, University of Missouri, Columbia, United States.
Michael S ChapmanDepartment of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, United States.

Funding

Adeno-Associated Virus Gene Therapy Vectors: Molecular Interactions on Cell EntryR35GM122564 · NIGMS · UNIVERSITY OF MISSOURI-COLUMBIA · PI MICHAEL S. CHAPMAN · 2017 to 2026
$6.7M
Structure-Function of AAV - a Viral Gene Therapy Vector.R01GM066875 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI CHAPMAN, MICHAEL S. · 2003 to 2016
$4.1M
NIGMS NIH HHS R01 GM066875NIGMS NIH HHS R35 GM122564
6 · The paper itself

Abstract

Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for the production of: (a) the outer protein shells (virus-like particles or VLPs) for serotype 2 (AAV-2) and (b) two fragments from the binding ectodomain of AAV's cellular receptor, AAVR. His

Indexed as

AAVAAVRAdeno-associated virusCo-receptorGene therapyViral entryVirusVirus receptor

Identifiers

PMID33654738
PMCPMC7842819

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.