ArticleQuantitative imaging in medicine and surgery2021
Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy with switchable objective lenses.
Article in Quantitative imaging in medicine and surgery, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Versatile high-speed volumetric imaging from microscopic to macroscopic scale by self-adaptive oblique plane microscopy.bioRxiv : the preprint server for biology · 2026Article
- Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity.Nature communications · 2023Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- Mesoscopic oblique plane microscopy with a diffractive light-sheet for large-scale 4D cellular resolution imaging.Optica · 2022Article
- Increasing the field-of-view in oblique plane microscopy via optical tiling.Biomedical optics express · 2022Article
- Review of data processing of functional optical microscopy for neuroscience.Neurophotonics · 2022Review
- Non-interferometric volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy.Biomedical optics express · 2022Article
- Scattering oblique plane microscopy forBiomedical optics express · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
backgroundConventional light sheet fluorescence microscopy (LSFM), or selective plane illumination microscopy (SPIM), enables high-resolution 3D imaging over a large volume by using two orthogonally aligned objective lenses to decouple excitation and emission. The recent development of oblique plane microscopy (OPM) simplifies LSFM design with only one single objective lens, by using off-axis excitation and remote focusing. However, most reports on OPM have a limited microscopic field of view (FOV), typically within 1×1 mm
methodsWe implemented an optical design of mesoscopic scanning OPM to allow the use of low numerical aperture (NA) objective lenses. The angle of the intermediate image before the remote focusing system was increased by a demagnification under Scheimpflug condition such that the light collecting efficiency in the remote focusing system was significantly improved. A telescope composed of cylindrical lenses was used to correct the distorted image caused by the demagnification design. We characterized the 3D resolutions and imaging volume by imaging fluorescent microspheres, and demonstrated the volumetric imaging on intact whole zebrafish larvae, mouse cortex, and multiple
resultsWe demonstrate a mesoscopic FOV up to ~6×5×0.6 mm
conclusionsThe proposed mesoscopic scanning OPM allows using low NA objectives such that centimeter-level FOV volumetric imaging can be achieved. With the extended FOV, simple sample mounting protocol, and the versatility of changeable FOVs/resolutions, our system will be ready for the varieties of applications requiring in vivo volumetric imaging over large length scales.
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