ArticleScientific reports2021
Selection of reference genes for gene expression analysis in Liriodendron hybrids' somatic embryogenesis and germinative tissues.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Characteristics and Expression Profiles of IdentifiedInternational journal of molecular sciences · 2025Article
- Genomic survey and expression analysis of LcARFs reveal multiple functions to somatic embryogenesis in Liriodendron.BMC plant biology · 2024Article
- Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis inForestry research · 2024Article
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- Characterization of theGenes · 2023Article
- Identification and validation of reference genes in vetiver (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Genome-Wide Analysis and Abiotic Stress-Responsive Patterns ofPlants (Basel, Switzerland) · 2023Article
- Selection of reference genes for RT-qPCR analysis in developing chicken embryonic ovary.Molecular biology reports · 2023Article
- Transcriptome profiling of high and low somatic embryogenesis rate of oil palm (Frontiers in plant science · 2023Article
- Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress.BMC plant biology · 2022Article
- Characterization of theFrontiers in plant science · 2021Article
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4 authors.
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Abstract
The differential expression of genes is crucial for plant somatic embryogenesis (SE), and the accurate quantification of gene expression levels relies on choosing appropriate reference genes. To select the most suitable reference genes for SE studies, 10 commonly used reference genes were examined in synchronized somatic embryogenic and subsequent germinative cultures of Liriodendron hybrids by using quantitative real-time reverse transcription PCR. Four popular normalization algorithms: geNorm, NormFinder, Bestkeeper and Delta-Ct were used to select and validate the suitable reference genes. The results showed that elongation factor 1-gamma, histone H1 linker protein, glyceraldehyde-3-phosphate dehydrogenase and α-tubulin were suitable for SE tissues, while elongation factor 1-gamma and actin were best for the germinative organ tissues. Our work will benefit future studies of gene expression and functional analyses of SE in Liriodendron hybrids. It is also serves as a guide of reference gene selection in early embryonic gene expression analyses for other woody plant species.
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