Evidence map›Paper›PMID 33653719›Full record

ArticleThe Journal of experimental biology2021

CRISPR/Cas9 mutagenesis reveals a role for ABCB1 in gut immune responses to Vibrio diazotrophicus in sea urchin larvae.

Travis J Fleming, Catherine S Schrankel, Himanshu Vyas, Hannah D Rosenblatt, Amro Hamdoun

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of experimental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Article
  3. The roles of ABCB1/P-glycoprotein drug transporters in regulating gut microbes and inflammation: insights from animal models, old and new.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024
    Review
  4. Semi-automated, high-content imaging of drug transporter knockout sea urchin (Lytechinus pictus) embryos.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. CRISPR/Cas9 knockin methodology for the sea urchin embryo.Molecular reproduction and development · 2023
    Article
  10. Article
  11. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Travis J FlemingCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA.
Catherine S SchrankelCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA.
Himanshu VyasCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA.
Hannah D RosenblattDepartment of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Amro HamdounCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA.
Scripps Institution of Oceanography · USHarvard University · USStanford University · US

Funding

Identity, mechanisms and early life impacts of transporter interfering compoundsR01ES027921 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHANG, GEOFFREY A, HAMDOUN, AMRO M · 2018 to 2022
$3.0M
Molecular Mechanisms of Marine Organohalogen Bioaccumulation and NeurotoxicityR01ES030318 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAMDOUN, AMRO M, PESSAH, ISAAC N · 2018 to 2022
$826k
Identifying the ABCs of xenobiotic metabolism in protecting the germ line lineageF32ES029843 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SCHRANKEL, CATHERINE SARAH · 2018 to 2022
$238k
NIEHS NIH HHS F32 ES029843NIEHS NIH HHS R01 ES027921NIEHS NIH HHS R01 ES030318
6 · The paper itself

Abstract

The ABC transporter ABCB1 plays an important role in the disposition of xenobiotics. Embryos of most species express high levels of this transporter in early development as a protective mechanism, but its native substrates are not known. Here, we used larvae of the sea urchin Strongylocentrotus purpuratus to characterize the early life expression and role of Sp-ABCB1a, a homolog of ABCB1. The results indicate that while Sp-ABCB1a is initially expressed ubiquitously, it becomes enriched in the developing gut. Using optimized CRISPR/Cas9 gene editing methods to achieve high editing efficiency in the F0 generation, we generated ABCB1a crispant embryos with significantly reduced transporter efflux activity. When infected with the opportunistic pathogen Vibrio diazotrophicus, Sp-ABCB1a crispant larvae demonstrated significantly stronger gut inflammation, immunocyte migration and cytokine Sp-IL-17 induction, as compared with infected control larvae. The results suggest an ancestral function of ABCB1 in host-microbial interactions, with implications for the survival of invertebrate larvae in the marine microbial environment.

Indexed as

CRISPR-Cas SystemsSea UrchinsAnimalsImmunityLarvaMutagenesisVibrioABCB1ABC transporterCRISPR/Cas9GastrulationGut epithelial immunityIL-17Marine larvaeSea urchinVibrio

Identifiers

PMID33653719
PMCPMC8077557
OpenAlexW3133807350

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.