ArticleMicrobial cell factories2021
Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 50 citations in OpenAlex.
- Harnessing prokaryotic amino acid transporters for metabolic engineering: mechanisms and biotechnological applications.Synthetic and systems biotechnology · 2026Review
- A short peptide derived from late embryogenesis abundant proteins enhances acid tolerance in Escherichia coli via modulation of two-component regulatory systems.The FEBS journal · 2026Article
- Whey as an Environmental Issue and Its Possible Solutions: Its Utilization as Culture Medium to Produce L-Threonine ThroughBiochemistry research international · 2026Review
- Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.Biotechnology for biofuels and bioproducts · 2024Article
- Engineering of increased L-Threonine production in bacteria by combinatorial cloning and machine learning.Metabolic engineering communications · 2023Article
- Microbial Secondary Metabolites via Fermentation Approaches for Dietary Supplementation Formulations.Molecules (Basel, Switzerland) · 2023Review
- l-Threonine production from whey and fish hydrolysate byHeliyon · 2023Article
- L-threonine promotes healthspan by expediting ferritin-dependent ferroptosis inhibition in C. elegans.Nature communications · 2022Article
- Toxicity and inhibition mechanism of gallic acid on physiology and fermentation performance of Escherichia coli.Bioresources and bioprocessing · 2022Article
- Improving CoQMicrobial cell factories · 2021Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBetaine, an osmoprotective compatible solute, has been used to improve L-threonine production in engineered Escherichia coli L-threonine producer. Betaine supplementation upregulates the expression of zwf encoding glucose-6-phosphate dehydrogenase, leading to the increase of NADPH, which is beneficial for L-threonine production. In E. coli, betaine can be taken through ProP encoded by proP or ProVWX encoded by proVWX. ProP is a H
resultsIn this study, the influence of ProP, ProVWX and betABIT on L-threonine production in E. coli has been investigated. Addition of betaine slightly improved the growth of the L-threonine producing E. coli strain TWF001 as well as the L-threonine production. Deletion of betABIT retarded the growth of TWF001 and slightly decreased the L-threonine production. However, deletion of proP or/and proVWX significantly increased the L-threonine production. When proP was deleted, the L-threonine production increased 33.3%; when proVWX was deleted, the L-threonine production increased 40.0%. When both proP and proVWX were deleted, the resulting strain TSW003 produced 23.5 g/l L-threonine after 36 h flask cultivation. The genes betABIT, proC, fadR, crr and ptsG were individually deleted from TSW003, and it was found that further absence of either crr (TWS008) or ptsG (TWS009) improved L-threonine production. TSW008 produced 24.9 g/l L-threonine after 36 h flask cultivation with a yield of 0.62 g/g glucose and a productivity of 0.69 g/l/h. TSW009 produced 26 g/l L-threonine after 48 h flask cultivation with a yield of 0.65 g/g glucose and a productivity of 0.54 g/l/h, which is 116% increase compared to the control TWF001.
conclusionsIn this study, L-threonine-producing E. coli strains TSW008 and TSW009 with high L-threonine productivity were developed by regulating the intracellular osmotic pressure. This strategy could be used to improve the production of other products in microorganisms.
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