Conversion of Racemic Unnatural Amino Acids to Optically Pure Forms by a Coupled Enzymatic Reaction.

Hannae Lee, Dongchan Kim, Sooin Kim, Hyun Soo Lee

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

The D-amino acid oxidase-carbon nanotubes: evaluation of cytotoxicity and biocompatibility of a potential anticancer nanosystem.3 Biotech · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Hannae LeeDepartment of Chemistry, Sogang University, 35 Baekbeomro Mapogu, Seoul 121-742, Korea.

Dongchan KimDepartment of Chemistry, Sogang University, 35 Baekbeomro Mapogu, Seoul 121-742, Korea.

Sooin KimDepartment of Chemistry, Sogang University, 35 Baekbeomro Mapogu, Seoul 121-742, Korea.ORCID 0000-0002-3694-3516

Hyun Soo LeeDepartment of Chemistry, Sogang University, 35 Baekbeomro Mapogu, Seoul 121-742, Korea.ORCID 0000-0002-1981-0391

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic code expansion (GCE) technology is a useful tool for the site-specific modification of proteins. An unnatural amino acid (UAA) is one of the essential components of this technique, typically required at high concentration (1 mM or higher) in growth medium. The supply of UAAs is an important limitation to the application of GCE technology, as many UAAs are either expansive or commercially unavailable. In this study, two UAAs in a racemic mixture were converted into optically pure forms using two enzymes, the d-amino acid oxidase (RgDAAO) from

Indexed as

Protein EngineeringAmino AcidsCloning, MolecularCulture MediaEscherichia coliGenetic CodeProteinsRhodotorulaSubstrate SpecificityAmino AcidsCulture MediaProteinsaminotransferasesD-amino acid oxidasesgenetic code expansionunnatural amino acids

Identifiers

PMID33652889

DOI10.3390/molecules26051274

PMCPMC7956486

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.