ArticlePoultry science2021
Sexing chickens (Gallus gallus domesticus) with high-resolution melting analysis using feather crude DNA.
Article in Poultry science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Association and variation in performance, nutrient digestibility, carcass traits, and intestinal development of broiler chickens by various male-to-female ratios.Veterinary and animal science · 2026Article
- Analysis of Transcriptome and Differentially Expressed Genes in Chicken Primordial Germ Cells.Animals : an open access journal from MDPI · 2026Article
- The spindlin gene as a universal marker for molecular sex identification in different poultry species.Poultry science · 2026Article
- Detection of non-invasive sexing of early chick embryos in intact eggs using laser speckle contrast imaging and deep neural networks.PloS one · 2026Article
- Cross-Species Validation of Pigeon-Specific CHD1 Primers for Molecular Sexing in Pet Birds.International journal of molecular sciences · 2025Article
- Effects of low-purine diet supplemented with Sida acuta Burm. f. on growth performance, purine deposition, and biomolecules in slow-growing chickens.Animal bioscience · 2025Article
- Effect of different ratios of male-to-female in broilers on performance and nutrients digestibility.Veterinary research forum : an international quarterly journal · 2025Article
- Sex Identification of a Multispecies Carinatae Birds by Chicken EE0.6 Gene Using Real-Time Recombinase-Aid Amplification Assay.Ecology and evolution · 2024Article
- Effects of Dietary Protein Levels, Net Energy Levels, and Essential Amino Acid-to-True Protein Ratios on Broiler Performance.Animals : an open access journal from MDPI · 2024Article
- Sex identification of ducklings based on acoustic signals.Poultry science · 2024Article
- Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed SheepAnimals : an open access journal from MDPI · 2022Article
- Potential of a mixture of eugenol and garlic tincture to improve performance and intestinal health in broilers under necrotic enteritis challenge.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- A Microencapsulated Mixture of Eugenol and Garlic Tincture Supplementation Mitigates the Effect of Necrotic Enteritis on Intestinal Integrity and Increases Goblet Cells in Broilers.Microorganisms · 2021Article
- Multi-carbohydrase enzymes improve feed energy in broiler diets containing standard or low crude protein.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of sex in broiler chickens allows researchers to reduce the level of variation in an experiment caused by the sex effect. Broiler breeds commonly used in research are no longer feather sexable because of the change in their genetics. Other alternate sexing methods are costly and difficult to apply on a large scale. Therefore, a sexing method is required that is both cost effective and highly sensitive as well as having the ability to offer high throughput genotyping. In this study, high-resolution melting (HRM) analysis was used to detect DNA variations present in the gene chromodomain helicase DNA binding 1 protein (CHD1) on the Z and W chromosomes (CHD1Z and CHD1W, respectively) of chickens. In addition, a simplified DNA extraction protocol, which made use of the basal part of chicken feathers, was developed to speed up the sexing procedure. Three pairs of primers, that is, CHD1UNEHRM1F/R, CHD1UNEHRM2F/R, and CHD1UNEHRM3F/R, flanking the polymorphic regions between CHD1Z and CHD1W were used to differentiate male and female chickens via distinct melting curves, typical of homozygous or heterozygous genotypes. The assay was validated by the HRM-sexing of 1,318 broiler chicks and verified by examining the sex of the birds after dissection. This method allows for the sexing of birds within a couple of days, which makes it applicable for use on a large scale such as in nutritional experiments.
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