Evidence map›Paper›PMID 33652016›Full record

ArticleJournal of virological methods2021

Cross comparison of AmpFire HPV genotyping assay and Roche human papillomavirus (HPV) linear array for HPV genotyping of anal swab samples.

Kaleigh A Connors, Stephen Abbott, Kamwing Jair, Jason Daniels, Madison Lintner, David Klein, Avery Wimpleberg, Jeanne A Jordan

Open access · greenAbstract read
In one paragraph

Article in Journal of virological methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Kaleigh A ConnorsDepartment of Epidemiology, School of Public Health, The George Washington University, Washington, DC, USA.
Stephen AbbottWhitman-Walker Health, Washington, DC, USA.
Kamwing JairDepartment of Epidemiology, School of Public Health, The George Washington University, Washington, DC, USA.
Jason DanielsBloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Madison LintnerDepartment of Epidemiology, School of Public Health, The George Washington University, Washington, DC, USA.
David KleinWhitman-Walker Health, Washington, DC, USA.
Avery WimplebergWhitman-Walker Health, Washington, DC, USA.
Jeanne A JordanDepartment of Epidemiology, School of Public Health, The George Washington University, Washington, DC, USA. Electronic address: jajordan@gwu.edu.
George Washington University · USWhitman-Walker Health · USJohns Hopkins University · US

Funding

Evaluating Companion Diagnostics to the anal Pap test to improve prediction of AIN2+ in HIV-infected MSMR01CA203604 · NCI · GEORGE WASHINGTON UNIVERSITY · PI JORDAN, JEANNE ANN · 2017 to 2021
$2.0M
NCI NIH HHS R01 CA203604
6 · The paper itself

Abstract

Although anal cancers represent just 0.5 % of all new cancer cases in U.S., rates have increased markedly, with highest rates in HIV-infected MSM. American Cancer Society estimates there will be ∼9090 new cases and ∼1420 deaths in 2021. We compared Roche Linear Array HPV Genotyping (Roche) and AmpFire HPV Genotyping (AmpFire) assays for concordance and agreement to detect 15 hr-HPV types from 151 anal specimens. Within run precision of AmpFire was assessed on 50 anal specimens. Specimens with Roche Combo-positive and HPV33, HPV35 and/or HPV58-positive results were further tested using HPV52-specific TaqMan assay. AmpFire generated valid results on 149/151 (98.7 %) specimens; 135/149 (90.6 %) and 134/149 (89.9 %) had detectable HR-HPV DNA by AmpFire or Roche, respectively. Overall concordance was 89.8 % (2007/2235, κ = 0.65). HPV16 showed highest overall concordance at 93.3 % (139/149, κ = 0.84). HPV68 had lowest overall concordance at 77.2 % (115/149, κ = 0.28). Kappa values were interpreted as being moderate or good for all other HR-HPV types. Within run precision generated 744/750 concordant results; R

Indexed as

AlphapapillomavirusPapillomavirus InfectionsSexual and Gender MinoritiesDNA, ViralGenotypeHomosexuality, MaleHumansMalePapillomaviridaeDNA, ViralAnal specimensGenotypingHPV

Identifiers

PMID33652016
PMCPMC8058289
OpenAlexW3133711671

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.