Evidence map›Paper›PMID 33651330›Full record

ArticleFood and environmental virology2021

Norovirus Extraction from Frozen Raspberries Using Magnetic Silica Beads.

Philippe Raymond, Sylvianne Paul, André Perron, Louise Deschênes

Open access · hybridAbstract readEvaluation Study
In one paragraph

Article in Food and environmental virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Philippe RaymondCanadian Food Inspection Laboratory (CFIA), St. Hyacinthe Laboratory, Food Virology, Saint Hyacinthe, QC, Canada. philippe.raymond@canada.ca.ORCID 0000-0001-9590-8975
Sylvianne PaulCanadian Food Inspection Laboratory (CFIA), St. Hyacinthe Laboratory, Food Virology, Saint Hyacinthe, QC, Canada.
André PerronCanadian Food Inspection Laboratory (CFIA), St. Hyacinthe Laboratory, Food Virology, Saint Hyacinthe, QC, Canada.
Louise DeschênesAgriculture and Agri-Food Canada (AAFC), St. Hyacinthe Research and Development Centre, Saint Hyacinthe, QC, Canada.
Cegep de Saint Hyacinthe · CAAgriculture and Agri-Food Canada · CACanadian Food Inspection Agency · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human noroviruses (HuNoV) are among the main causes of acute gastroenteritis worldwide. Frozen raspberries have been linked to several HuNoV food-related outbreaks. However, the extraction of HuNoV RNA from frozen raspberries remains challenging. Recovery yields are low, and real-time quantitative reverse transcriptase PCR (RT-qPCR) inhibitors limit the sensitivity of the detection methodologies. A new approach using fine magnetic silica beads was developed for the extraction of HuNoV spiked on frozen raspberries. Relatively low recovery yields were observed with both the magnetic silica bead and the reference ISO 15216-1:2017 methods. High RT-qPCR inhibition was observed with the ISO 15216-1:2017 recommended amplification kit but could be reduced by using an alternative kit. Reducing RT-qPCR inhibition is important to limit the number of inconclusive HuNoV assays thus increasing the capacity to assess the HuNoV prevalence in frozen raspberries.

Indexed as

FruitGastroenteritisHumansImmunomagnetic SeparationNorovirusReal-Time Polymerase Chain ReactionRNA, ViralRubusSilicon DioxideRNA, ViralSilicon DioxideNorovirusRaspberriesRNA extractionRT-qPCRSilica

Identifiers

PMID33651330
PMCPMC8116234
OpenAlexW3135143584

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.