ArticleMethods in molecular biology (Clifton, N.J.)2021
Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Bifidobacterium pseudocatenulatum capsular exopolysaccharide enhances systemic anti-tumour immunity in pre-clinical breast cancer.Research square · 2026Article
- Postbiotics: An Alternative for Improving Health and Performance of Poultry Production.Microorganisms · 2025Review
- Taxonomic and phenotypic analysis of bifidobacteria isolated from IBD patients as potential probiotic strains.BMC microbiology · 2024Article
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Authors and funding
1 author.
Funding
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Abstract
This chapter describes some of the available methods to assess EPS production in bifidobacteria, being largely based on those developed for the same purpose for members of the lactic acid bacteria group. The first step is detection of putative EPS-producing bifidobacteria based on a mucoid and/or ropy phenotype. Next, a basic procedure is described for the isolation of the glycan polymer based on the release from bifidobacterial cells grown and collected from the surface of agar-MRSc ("crude EPS"), followed by a purification procedure intended to remove other bacterial macromolecules (DNA and proteinaceous material) to generate "purified EPS." Finally, several methods used for quantification and physical-chemical characterization of isolated/purified polysaccharide are outlined.
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