Evidence map›Paper›PMID 33649811›Full record

Observational studyOncology reports2021

Roles of the H19/microRNA‑675 axis in the proliferation and epithelial‑mesenchymal transition of human cutaneous squamous cell carcinoma cells.

Wenqing Zhang, Kaili Zhou, Xue Zhang, Chenglong Wu, Dan Deng, Zhirong Yao

Erratum issuedOpen access · hybridAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Oncology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
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  9. Long noncoding RNAs in ubiquitination, protein degradation, and human diseases.Biochimica et biophysica acta. Gene regulatory mechanisms · 2024
    Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Article
  16. Long noncoding RNA (lncRNA)Genes & diseases · 2023
    Review
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  19. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Wenqing Zhang *Department of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.
Kaili Zhou *Department of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.
Xue ZhangDepartment of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.
Chenglong WuDepartment of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.
Dan DengDepartment of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.
Zhirong YaoDepartment of Dermatology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.
Shanghai Jiao Tong University · CNXinHua Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The long non‑coding RNA (lncRNA) H19 and microRNA(miR)‑675 were reported to serve an important role in the tumorigenesis and metastasis of numerous cancer types by promoting the epithelial‑mesenchymal transition (EMT) process; however, the underlying mechanisms of action of H19 and miR‑675 in cutaneous squamous cell carcinoma (cSCC) remain unknown. The mRNA expression levels of H19 and miR‑675 were analyzed using reverse transcription‑quantitative PCR, and Cell Counting Kit‑8, wound healing and Transwell assays were performed to analyze the cell proliferation, migration and invasion of cSCC cells, respectively. The levels of cell apoptosis were also determined using a TUNEL assay. Protein expression levels of p53 and marker proteins related to the EMT process were analyzed using western blotting. In addition, a dual luciferase reporter assay was performed to determine the interactions between H19, miR‑675 and p53. The results of the present study revealed that the expression levels of H19 and miR‑675 were upregulated in cSCC tissues and cSCC cell lines. The knockdown of H19 or miR‑675 expression inhibited cell proliferation, migration and invasion, but induced cell apoptosis. In addition, the expression levels of EMT‑related markers were also downregulated. The overexpression of H19 upregulated the expression levels of its predicted target, miR‑675, which subsequently promoted the EMT process and downregulated the expression levels of p53. Conversely, the genetic silencing of H19 or miR‑675 inhibited proliferation and invasion in SCL1 and A431 cSCC cell lines. In conclusion, the findings of the present study provided novel insight into the potential role of H19 and miR‑675 in the development, metastasis and progression of cSCC, which may help the development of treatments for cSCC.

Indexed as

AdultAgedAged, 80 and overCarcinoma, Squamous CellCell Line, TumorCell ProliferationEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansMaleMicroRNAsMiddle AgedNeoplasm InvasivenessRNA, Long NoncodingH19 long non-coding RNAMicroRNAsMIRN675 microRNA, humanRNA, Long NoncodingTP53 protein, humanTumor Suppressor Protein p53cutaneous squamous cell carcinomaepithelial‑mesenchymal transitionlong non‑coding RNA H19microRNA‑675p53

Identifiers

PMID33649811
PMCPMC7905556
OpenAlexW3131688059

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.