ArticleThe ISME journal2021
Experimental identification and in silico prediction of bacterivory in green algae.
Article in The ISME journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Dissolved Organic Carbon Regulates Bacterial Ingestion by Tetraselmis sp.Microbial ecology · 2026Article
- Energy metabolism and adaptation to hypoxia in the non-photosynthetic green alga Leontynka.BMC biology · 2026Article
- Metatranscriptomics reveals protistan and viral metabolic reprogramming of sinking particle microbiomes in Oyashio waters.ISME communications · 2026Article
- Conserved tripartite tail proteins mediate virophage-host interactions throughJournal of virology · 2025Article
- Nuclear genome assembly and annotation for the phagotrophic green alga Nephroselmis pyriformis.Journal of phycology · 2025Article
- Metatranscriptomes-based sequence similarity networks uncover genetic signatures within parasitic freshwater microbial eukaryotes.Microbiome · 2025Article
- Latitude- and depth-driven divergence in protist trophic strategies revealed by a machine learning model.Frontiers in microbiology · 2025Article
- Do trophic strategies shape biogeography and environmental niches? Marine dinoflagellates as a case study.ISME communications · 2025Article
- Proliferation of a bloom-forming phytoplankton via uptake of polyphosphate-accumulating bacteria under phosphate-limiting conditions.ISME communications · 2025Article
- Protists and protistology in the Anthropocene: challenges for a climate and ecological crisis.BMC biology · 2024Review
- Prevalence and Preferred Niche of Small Eukaryotes with Mixotrophic Potentials in the Global Ocean.Microorganisms · 2024Article
- Transcriptomics reveal a unique phago-mixotrophic response to low nutrient concentrations in the prasinophyteISME communications · 2024Article
- Long-Read-Based Genome Assembly Reveals Numerous Endogenous Viral Elements in the Green Algal Bacterivore Cymbomonas tetramitiformis.Genome biology and evolution · 2023Article
- Phagocytosis underpins the biotrophic lifestyle of intracellular parasites in the class Phytomyxea (Rhizaria).The New phytologist · 2023Article
- Convergent evolution and horizontal gene transfer in Arctic Ocean microalgae.Life science alliance · 2023Article
- Trophic strategies explain the ocean niches of small eukaryotic phytoplankton.Proceedings. Biological sciences · 2023Article
- Metagenome assembled genomes are for eukaryotes too.Cell genomics · 2022Article
- The genome of a nonphotosynthetic diatom provides insights into the metabolic shift to heterotrophy and constraints on the loss of photosynthesis.The New phytologist · 2021Article
- Unstable Relationship BetweenFrontiers in plant science · 2021Article
- Mixoplankton and mixotrophy: future research priorities.Journal of plankton researchReview
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While algal phago-mixotrophs play a major role in aquatic microbial food webs, their diversity remains poorly understood. Recent studies have indicated several species of prasinophytes, early diverging green algae, to be able to consume bacteria for nutrition. To further explore the occurrence of phago-mixotrophy in green algae, we conducted feeding experiments with live fluorescently labeled bacteria stained with CellTracker Green CMFDA, heat-killed bacteria stained with 5-(4,6-dichlorotriazin-2-yl) aminofluorescein (DTAF), and magnetic beads. Feeding was detected via microscopy and/or flow cytometry in five strains of prasinophytes when provided with live bacteria: Pterosperma cristatum NIES626, Pyramimonas parkeae CCMP726, Pyramimonas parkeae NIES254, Nephroselmis pyriformis RCC618, and Dolichomastix tenuilepis CCMP3274. No feeding was detected when heat-killed bacteria or magnetic beads were provided, suggesting a strong preference for live prey in the strains tested. In parallel to experimental assays, green algal bacterivory was investigated using a gene-based prediction model. The predictions agreed with the experimental results and suggested bacterivory potential in additional green algae. Our observations underline the likelihood of widespread occurrence of phago-mixotrophy among green algae, while additionally highlighting potential biases introduced when using prey proxy to evaluate bacterial ingestion by algal cells.
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