ArticleScientific reports2021
Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.Biotechnology and bioengineering · 2026Review
- Algal and Cyanobacterial Hydrolysates as Serum-Reducing Supplements Across Mammalian, Avian and Fish Cells with Identification of Bioactive Chromopeptides.Foods (Basel, Switzerland) · 2026Article
- Development and characterization of red seabream (Pagrus major) muscle satellite cell lines for cultivated seafood: highlighting serum reduction and microcarrier expansion.In vitro cellular & developmental biology. Animal · 2026Article
- Selective Quenching of Peracetic Acid by Sodium Dithionite Enables Rapid, Non-Thermal Sterilization forMicroorganisms · 2026Article
- Inhibition of free radicals and inflammation on RAW264.7 macrophage cell line by Arthrospira platensis extract.Scientific reports · 2025Article
- Systematic evaluation of porcine platelet lysate as a fetal bovine serum substitute: enhancing cell proliferation, maintaining differentiation potential, and ensuring immunophenotypic stability.Stem cell research & therapy · 2025Article
- The potential ofRegenerative therapy · 2025Article
- Spirulina Unveiled: A Comprehensive Review on Biotechnological Innovations, Nutritional Proficiency, and Clinical Implications.Current pharmaceutical biotechnology · 2025Review
- Screening Algal and Cyanobacterial Extracts to Identify Potential Substitutes for Fetal Bovine Serum in Cellular Meat Cultivation.Foods (Basel, Switzerland) · 2024Article
- Eco-friendlyHeliyon · 2024Article
- Serum-Free Medium Supplemented withFoods (Basel, Switzerland) · 2024Article
- Serum-Free Media Formulation Using Marine Microalgae Extracts and Growth Factor Cocktails for Madin-Darby Canine Kidney and Vero Cell Cultures.International journal of molecular sciences · 2024Article
- Preliminary study on comparison of egg extraction methods for development of fetal bovine serum substitutes in cultured meat.Food chemistry: X · 2024Article
- Artificial intelligence and machine learning applications for cultured meat.Frontiers in artificial intelligence · 2024Review
- Enhanced Photosynthetic Pigment Production Using a Scaled-Up Continuously Circulated Bioreactor.Marine drugs · 2023Article
- Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production.Scientific reports · 2023Article
- Efficient Myogenic/Adipogenic Transdifferentiation of Bovine Fibroblasts in a 3D Bioprinting System for Steak-Type Cultured Meat Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Wide Range Applications of Spirulina: From Earth to Space Missions.Marine drugs · 2022Review
- Synthetic biology in marine cyanobacteria: Advances and challenges.Frontiers in microbiology · 2022Review
- Development and evaluation of culture media based on extracts of the cyanobacteriumFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an ethical debate regarding the collection process, lack of standardized ingredients, and high cost. Herein, therefore, we evaluated the possibility of using edible cyanobacterium (Spirulina maxima), which is a nutrient-rich, sustainable, and ethically acceptable source, as a novel substitute for fetal bovine serum (FBS). H460 cells were cultured to the 10th generation by adding a mixture of spirulina animal cell culture solution (SACCS) and FBS to the culture medium. Cell morphology and viability, cell cycle, apoptosis, proteomes, and transcriptomes were assessed. We observed that SACCS had better growth-promoting capabilities than FBS. Cell proliferation was promoted even when FBS was replaced by 50-70% SACCS; there was no significant difference in cell shape or viability. There were only slight differences in the cell cycle, apoptosis, proteomes, and transcriptomes of the cells grown in presence of SACCS. Therefore, SACCS has the potential to be an effective, low-cost, and eco-friendly alternative to FBS in in vitro culture.
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