ArticleProceedings of the National Academy of Sciences of the United States of America2021
Lipid droplets in mammalian eggs are utilized during embryonic diapause.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 69 citations in OpenAlex.
- Metabolic regulation of oocyte maturation and early embryo development in mammals.Life metabolism · 2026Review
- Strain- and lineage-specific regulation of lipid droplet metabolism in the mouse blastocyst†.Biology of reproduction · 2026Article
- Putting mammalian early embryonic cells into dormancy.Nature protocols · 2026Review
- Metabolic hubs in reproduction: The regulatory network of lipid droplets in gamete and embryo physiology (Review).International journal of molecular medicine · 2026Review
- Article
- ChREBPα: a central metabolic sensor driving lipid droplet renewal in preimplantation mouse embryos.Frontiers in cell and developmental biology · 2026Article
- Using diapause as a platform to understand the biology of dormancy.Open biology · 2025Review
- Agouti-signaling protein (ASIP) improves bovine in vitro matured oocyte developmental competence and modulates lipid content.Journal of assisted reproduction and genetics · 2025Article
- Raman analysis of lipids in cells: Current applications and future prospects.Journal of pharmaceutical analysis · 2025Review
- Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging.Chemical & biomedical imaging · 2025Article
- Roe deer uterine fluid metabolome reveals elevated glycolysis, fatty acid breakdown, and spermidine synthesis upon reactivation from diapause†.Biology of reproduction · 2025Article
- Non-invasive applications of Raman spectroscopy in assisted reproduction.Frontiers in endocrinology · 2025Review
- Female-age-dependent changes in the lipid fingerprint of the mammalian oocytes.Human reproduction (Oxford, England) · 2024Article
- Article
- A mitochondrial redox switch licenses the onset of morphogenesis in animals.bioRxiv : the preprint server for biology · 2024Article
- TET activity safeguards pluripotency throughout embryonic dormancy.Nature structural & molecular biology · 2024Article
- Cpt1a Drives primed-to-naïve pluripotency transition through lipid remodeling.Communications biology · 2024Article
- Article
- The SWI/SNF ATP-dependent chromatin remodeling complex in cell lineage priming and early development.Biochemical Society transactions · 2024Review
- Lipid droplet formation is spatiotemporally regulated in oocytes during follicular development in mice.The Journal of reproduction and development · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic diapause (ED) is a temporary arrest of an embryo at the blastocyst stage when it waits for the uterine receptivity signal to implant. ED used by over 100 species may also occur in normally "nondiapausing" mammals when the uterine receptivity signal is blocked or delayed. A large number of lipid droplets (LDs) are stored throughout the preimplantation embryo development, but the amount of lipids varies greatly across different mammalian species. Yet, the role of LDs in the mammalian egg and embryo remains unknown. Here, using a mouse model, we provide evidence that LDs play a crucial role in maintaining ED. By mechanical removal of LDs from zygotes, we demonstrated that delipidated embryos are unable to survive during ED. LDs are not essential for normal prompt implantation, without ED. We further demonstrated that with the progression of ED, the amount of intracellular lipid reduces, and composition changes. This decrease in lipid is caused by a switch from carbohydrate metabolism to lipid catabolism in diapausing blastocysts, which also exhibit increased release of exosomes reflecting elevated embryonic signaling to the mother. We have also shown that presence of LDs in the oocytes of various mammals positively corelates with their species-specific length of diapause. Our results reveal the functional role of LDs in embryonic development. These results can help to develop diagnostic techniques and treatment of recurrent implantation failure and will likely ignite further studies in developmental biology and reproductive medicine fields.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.