SynthesisBMJ open gastroenterology2021
Accuracy and comparison of two rapid multiplex PCR tests for gastroenteritis pathogens: a systematic review and meta-analysis.
Synthesis in BMJ open gastroenterology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 3 syntheses or guidelines pooled it, 50 citations in OpenAlex.
- Diagnostic Accuracy of Multiplex NAAT/PCR and Culture AgainstPathogens (Basel, Switzerland) · 2025Pooled it
- Diagnostic accuracy of multiplex nucleic acid amplification tests forPathogens and global health · 2023Pooled it
- Meta-analysis of variable-temperature PCR technique performance for diagnosising Schistosoma japonicum infections in humans in endemic areas.PLoS neglected tropical diseases · 2022Pooled it
- Diagnostics for priority bacterial pathogens: global gaps and research needs for curbing antimicrobial resistance in low-resource settings.The Lancet. Microbe · 2026Review
- Impact of the BioFireBMC infectious diseases · 2026Article
- PCR-Based Versus Conventional Stool Testing in Hospitalized Patients with Diarrhea: Diagnostic Yield, Clinical Impact, and Stewardship Implications.Microorganisms · 2025Article
- Parent and Clinician Perspectives on Diagnostic Testing for Children With Diarrhea: A Qualitative Study.JAMA network open · 2025Article
- Article
- Prevalence and Genetic Diversity of Adenovirus 40/41, Astrovirus, and Sapovirus in Children With Acute Gastroenteritis in Kansas City, 2011-2016.The Journal of infectious diseases · 2025Article
- Multiplex PCR Detection of Enteric Pathogens in a Community-based Birth Cohort in Ecuador: Comparison of xTAG-GPP and TaqMan Array Card Assays.Open forum infectious diseases · 2025Article
- Article
- Etiological, Clinical, and Epidemiological Characteristics of Acute Viral Gastroenteritis in an Adult Population in a Tertiary Level Hospital in Spain.Infectious diseases and therapy · 2025Article
- Prospective evaluation of different faecal preservation media for travellers' diarrhoea diagnostic application with multiplex PCR BioFire FilmArray in resource-limited settings.Journal of medical microbiology · 2025Article
- Towards Meaningful Interpretation of Molecular Data: Insights Gained from HMMD Challenges inDiagnostics (Basel, Switzerland) · 2024Article
- Nanoplasmonic microarray-based solid-phase amplification for highly sensitive and multiplexed molecular diagnostics: application for detecting SARS-CoV-2.Mikrochimica acta · 2024Article
- Clinical Performance of the LiquidArrayDiagnostics (Basel, Switzerland) · 2024Article
- Multiplex Gastrointestinal Panel Testing in Hospitalized Patients With Acute Diarrhea in Thailand.Open forum infectious diseases · 2024Article
- The Gut-Wrenching Effects of Cryptosporidiosis and Giardiasis in Children.Microorganisms · 2023Review
- Rapid diagnostics to enhance therapy selection for the treatment of bacterial infections.Current pharmacology reports · 2023Article
- Viral infections in inflammatory bowel disease: Tips and tricks for correct management.World journal of gastroenterology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe primary aim is to provide a summary of evidence for the diagnostic accuracies of multiplex PCR gastrointestinal (GI) panels-BioFire FilmArray and Luminex xTAG on the detection of gastroenteritis pathogens. The secondary aim is to compare the performance of these GI panels head to head.
methodsA comprehensive search up to 1 December 2019 was conducted on PubMed, Embase, Ovid Medline and Web of Science for studies that used FilmArray or Luminex xTAG Gastrointestinal Pathogen Panel (GPP) for diagnosis of acute gastroenteritis. A summary of diagnostic accuracies for the 16 pathogens were calculated by comparing the GI panels to the current gold standards (conventional standard microbiology techniques such as culture or PCR for bacteria, PCR or enzyme immunoassay (EIA) for viruses, microscopy or EIA for parasite). Hierarchical summary receiver operating characteristic (HSROC) curve analysis, pretest and post-test probabilities were used for estimating the pathogen detection performance.
resultsA total of 11 studies with 7085 stool samples were eligible for analysis. Multiplex PCRs demonstrated high diagnostic accuracy, with specificity ≧0.98 and area under the ROC curve (AUROC) ≧0.97 for all the pathogens except for
conclusionsThis is the first meta-analysis that is a head-to-head comparison examining the performance of the novel multiplex PCR-based tests Luminex xTAG GPP and FilmArray GI panel in detecting each pathogen. Point estimates calculated from eligible studies showed that both GI panels are highly accurate and may provide important diagnostic information for early identification of gastroenteritis. In addition, although FilmArray has higher sensitivity and post-test probability than xTAG GPP for most of the pathogens, how this will translate to a clinical setting remains unclear.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.