Evidence map›Paper›PMID 33648601›Full record

ArticleStem cell research & therapy2021

Exosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and enhances adipogenic versus osteogenic differentiation in human bone marrow mesenchymal stem cells by sponging miR-431-5p.

Feng Zhi, Yi Ding, Rong Wang, Yujiao Yang, Kaiming Luo, Fei Hua

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 59 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed, 1 pooled it
6.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.

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  20. [Research Progress in the Regulatory Role of circRNA-miRNA Network in Bone Remodeling].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Feng Zhi *Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou City, 213003, Jiangsu, China. danielzhif@suda.edu.cn.ORCID http://orcid.org/0000-0002-3414-0162
Yi Ding *Department of Geriatrics, Third Affiliated Hospital of Soochow University, Changzhou City, 213003, Jiangsu, China.
Rong WangDepartment of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou City, 213003, Jiangsu, China.
Yujiao YangDepartment of Geriatrics, Third Affiliated Hospital of Soochow University, Changzhou City, 213003, Jiangsu, China.
Kaiming LuoDepartment of Endocrinology, Third Affiliated Hospital of Soochow University, Changzhou City, 213003, Jiangsu, China.
Fei HuaDepartment of Endocrinology, Third Affiliated Hospital of Soochow University, Changzhou City, 213003, Jiangsu, China. huafei1970@suda.edu.cn.
Soochow University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAs one of the most common chronic diseases in the world, osteoporosis occurs especially in postmenopausal women. Circular RNAs (circRNAs) are emerging as major drivers in human disease. The aim of the present study was to analyse circRNA expression profiles in osteoporosis and to explore the clinical significance and the regulatory molecular mechanism of hsa_circ_0006859 during osteoporosis.

methodsExosomes were isolated from clinically collected serum samples. A circRNA microarray was performed to screen differentially expressed circRNAs. Quantitative real-time PCR (qRT-PCR) and western blot were performed to analyse target gene mRNA expression and protein expression. Alizarin red staining (ARS) was performed to evaluate the mineralization ability of human bone marrow mesenchymal stem cells (hBMSCs). Oil Red O staining was performed to evaluate the lipid droplet formation ability of hBMSCs. Bioinformatics analysis and the luciferase reporter assay were performed to investigate the interaction between two genes.

resultsHsa_circ_0006859 was identified as one of the most upregulated circRNAs in the microarray analysis. Hsa_circ_0006859 in exosomes was upregulated in osteoporosis patients compared to healthy controls. Hsa_circ_0006859 differentiated osteopenia or osteoporosis patients from healthy controls with high sensitivity and specificity. Hsa_circ_0006859 suppressed osteoblastic differentiation and promoted adipogenic differentiation of hBMSCs. Hsa_circ_0006859 directly bound to miR-431-5p, and ROCK1 was identified as a novel target gene of miR-431-5p. Hsa_circ_0006859 is a competing endogenous RNA (ceRNA) of miR-431-5p that promotes ROCK1 expression. Hsa_circ_0006859 suppressed osteogenesis and promoted adipogenesis by sponging miR-431-5p to upregulate ROCK1.

conclusionsExosomal hsa_circ_0006859 is a potential biomarker for postmenopausal osteoporosis and controls the balance between osteogenesis and adipogenesis in hBMSCs by sponging miR-431-5p.

Indexed as

Mesenchymal Stem CellsMicroRNAsOsteoporosis, PostmenopausalAdipogenesisBiomarkersCell DifferentiationFemaleHumansOsteogenesisrho-Associated KinasesBiomarkersMicroRNAsMIRN431 microRNA, humanrho-Associated KinasesROCK1 protein, humanAdipogenesisExosomehsa_circ_0006859Human bone marrow mesenchymal stem cells (hBMSCs)OsteogenesisOsteoporosis

Identifiers

PMID33648601
PMCPMC7923524
OpenAlexW3133694188

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.