ArticleThe EMBO journal2021
Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension.
Article in The EMBO journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Specialisation of meiotic kinetochores revealed through a synthetic spindle assembly checkpoint strategy.eLife · 2026Article
- Hybrid female sterility due to cohesin protection errors in mouse oocytes.Science advances · 2026Article
- Protein-targeting reverse genetic approaches: the future of oocyte and preimplantation embryo research.Molecular human reproduction · 2025Review
- Heat application in live cell imaging.FEBS open bio · 2024Review
- Distinct roles of spindle checkpoint proteins in meiosis.Current biology : CB · 2024Article
- Age-dependent loss of cohesion protection in human oocytes.Current biology : CB · 2024Article
- Spo13/MEIKIN ensures a Two-Division meiosis by preventing the activation of APC/CThe EMBO journal · 2023Article
- Meiosis in budding yeast.Genetics · 2023Article
- Meiotic cells escape prolonged spindle checkpoint activity through kinetochore silencing and slippage.PLoS genetics · 2023Article
- Meiotic Cells Escape Prolonged Spindle Checkpoint Activity Through Premature Silencing and Slippage.bioRxiv : the preprint server for biology · 2023Article
- Review
- The Role of REC8 in the Innate Immune Response to Viral Infection.Journal of virology · 2022Article
- The Spo13/Meikin pathway confines the onset of gamete differentiation to meiosis II in yeast.The EMBO journal · 2022Article
- Targeted protein degradation in mammalian cells: A promising avenue toward future.Computational and structural biotechnology journal · 2022Review
- Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis II.The EMBO journal · 2021Article
- Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension.The EMBO journal · 2021Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome haploidization involves sequential loss of cohesin from chromosome arms and centromeres during two meiotic divisions. At centromeres, cohesin's Rec8 subunit is protected from separase cleavage at meiosis I and then deprotected to allow its cleavage at meiosis II. Protection of centromeric cohesin by shugoshin-PP2A seems evolutionarily conserved. However, deprotection has been proposed to rely on spindle forces separating the Rec8 protector from cohesin at metaphase II in mammalian oocytes and on APC/C-dependent destruction of the protector at anaphase II in yeast. Here, we have activated APC/C in the absence of sister kinetochore biorientation at meiosis II in yeast and mouse oocytes, and find that bipolar spindle forces are dispensable for sister centromere separation in both systems. Furthermore, we show that at least in yeast, protection of Rec8 by shugoshin and inhibition of separase by securin are both required for the stability of centromeric cohesin at metaphase II. Our data imply that related mechanisms preserve the integrity of dyad chromosomes during the short metaphase II of yeast and the prolonged metaphase II arrest of mammalian oocytes.
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