ArticleVeterinary world2021
Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera.
Article in Veterinary world, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- Epidemiological surveillance of BVDV, Pasteurella multocida, and Brucella spp. infections in cattle from Guizhou, China using a newly established triplex TaqMan qPCR assay.Veterinary research communications · 2026Article
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Large-scale evaluation of bacteriological-based method and qPCR performance for Brucellosis diagnosis in livestock using Bayesian latent class analysis.The veterinary quarterly · 2025Article
- Epidemiological baseline of Brucella spp. in South African wildlife.PLoS neglected tropical diseases · 2025Article
- Comparative Evaluation of Diagnostic Tests for Brucellosis in Humans and Animals: A Meta-Analytical Approach.Veterinary sciences · 2025Article
- Molecular evidence of Brucella abortus circulating in cattle, goats, and humans in Central Equatoria State, South Sudan.Scientific reports · 2025Article
- Establishment and application of a TaqMan-based quantitative PCR assay for simultaneous detection of bovineFrontiers in microbiology · 2025Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimDifferent polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of MATERIALS AND
methodsOne hundred and eighty-four serum samples were collected from bacteriologically positive and negative cows with ages ranging from 1 to 5 years old at some infected private farms in the Nile Delta under quarantine measures as well as brucellosis free farms. These samples were classified into four groups after serological diagnosis and investigated by TaqMan RT-PCR and conventional PCR targeting the IS711 gene for
resultsTaqMan RT-PCR revealed superiority over conventional PCR; it was able to detect
conclusionA cattle serum sample is not the metric of choice for targeting
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.