ArticleHorticulture research2021
Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
- A RUBY-Based Visual Hairy Root Transformation and CRISPR-Plants (Basel, Switzerland) · 2026Article
- Epigenetic orchestration of sugar signaling in plant development and stress adaptation.Horticulture research · 2026Article
- Overcoming breeding barriers with genome editing in autopolyploid crops.BMC plant biology · 2026Review
- Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Genomic resources and genetic improvement of vital tropical and subtropical fruit crops: current status and prospects.AoB PLANTS · 2025Review
- Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.Horticulture research · 2025Article
- CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit.BMC plant biology · 2025Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- A Truncated Endogenous U6 Promoter Enables High-Efficiency CRISPR Editing in Flax (Plants (Basel, Switzerland) · 2025Article
- Two TAL effectors of Xanthomonas citri promote pustule formation by directly repressing the expression of GRAS transcription factor in citrus.Molecular horticulture · 2025Article
- Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenousaBIOTECH · 2025Article
- Establishing a CRISPR/Cas9 genome editing framework in pigeonpea (Cajanus cajan L.) by targeting phytoene desaturase (PDS) gene disruption.Journal, genetic engineering & biotechnology · 2025Article
- CRISPR-Cas9-mediated deletions of FvMYB46 in Fragaria vesca reveal its role in regulation of fruit set and phenylpropanoid biosynthesis.BMC plant biology · 2025Article
- Advances in the molecular mechanism of grapevine resistance to fungal diseases.Molecular horticulture · 2025Review
- Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation.Frontiers in genome editing · 2025Review
- Development of an endogenous promoter-driven CRISPR/Cas9 system for genome editing inForestry research · 2025Article
- Evolution of agricultural biotechnology is the paradigm shift in crop resilience and development: a review.Frontiers in plant science · 2025Review
- Identification of U6 Promoter and Establishment of Gene-Editing System inPlants (Basel, Switzerland) · 2024Article
- Genome editing in cells of apple cultivar 'Fuji' using geminivirus-derived replicons for transient expression of CRISPR/Cas9 components.Plant biotechnology (Tokyo, Japan) · 2024Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
Abstract
The efficacy of the CRISPR/Cas9 system in grapevine (Vitis vinifera L.) has been documented, but the optimization of this system, as well as CRISPR/Cas9-mediated multiplex genome editing, has not been explored in this species. Herein, we identified four VvU3 and VvU6 promoters and two ubiquitin (UBQ) promoters in grapevine and demonstrated that the use of the identified VvU3/U6 and UBQ2 promoters could significantly increase the editing efficiency in grape by improving the expression of sgRNA and Cas9, respectively. Furthermore, we conducted multiplex genome editing using the optimized CRISPR/Cas9 vector that contained the conventional multiple sgRNA expression cassettes or the polycistronic tRNA-sgRNA cassette (PTG) by targeting the sugar-related tonoplastic monosaccharide transporter (TMT) family members TMT1 and TMT2, and the overall editing efficiencies were higher than 10%. The simultaneous editing of TMT1 and TMT2 resulted in reduced sugar levels, which indicated the role of these two genes in sugar accumulation in grapes. Moreover, the activities of the VvU3, VvU6, and UBQ2 promoters in tobacco genome editing were demonstrated by editing the phytoene desaturase (PDS) gene in Nicotiana benthamiana leaves. Our study provides materials for the optimization of the CRISPR/Cas9 system. To our knowledge, our simultaneous editing of the grape TMT family genes TMT1 and TMT2 constitutes the first example of multiplex genome editing in grape. The multiplex editing systems described in this manuscript expand the toolbox of grape genome editing, which would facilitate basic research and molecular breeding in grapevine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.