ArticleProtein science : a publication of the Protein Society2021
Measuring gas vesicle dimensions by electron microscopy.
Article in Protein science : a publication of the Protein Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Acoustic percolation switches enable targeted drug delivery controlled by diagnostic ultrasound.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Excavation of acoustic nanostructures biosynthesis gene clusters by combinatorial strategy.Advanced biotechnology · 2025Article
- Ultrafast optical and passive acoustic mapping characterization of nanoscale cavitation nuclei based on gas vesicle proteins.AIP advances · 2025Article
- UltrasensitiveAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Genetically engineered gas vesicle proteins with proliferative potential for synergistic targeted tumor therapy.RSC advances · 2025Article
- Harmonic imaging for nonlinear detection of acoustic biomolecules.APL bioengineering · 2024Article
- Pressure estimation of ultra-high frequency ultrasound using gas vesicles.The Journal of the Acoustical Society of America · 2024Article
- Advances in the application of gas vesicles in medical imaging and disease treatment.Journal of biological engineering · 2024Review
- Protein Gas Vesicles ofACS nano · 2024Article
- 50-nm Gas-Filled Protein Nanostructures to Enable the Access of Lymphatic Cells by Ultrasound Technologies.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Truly Tiny Acoustic Biomolecules for Ultrasound Imaging and Therapy.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Structural biology of microbial gas vesicles: historical milestones and current knowledge.Biochemical Society transactions · 2024Review
- Enhancing photothermal therapy of tumors with image-guided thermal control of gene-expressing bacteria.Theranostics · 2024Article
- Truly tiny acoustic biomolecules for ultrasound imaging and therapy.bioRxiv : the preprint server for biology · 2023Article
- Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET.Structure (London, England : 1993) · 2023Article
- Article
- Bacterial flotation devices enhance ultrasound imaging.Biophysical journal · 2022Article
- Geometric effects in gas vesicle buckling under ultrasound.Biophysical journal · 2022Article
- Multiplexed Ultrasound Imaging Using Spectral Analysis on Gas Vesicles.Advanced healthcare materials · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Gas vesicles (GVs) are cylindrical or spindle-shaped protein nanostructures filled with air and used for flotation by various cyanobacteria, heterotrophic bacteria, and Archaea. Recently, GVs have gained interest in biotechnology applications due to their ability to serve as imaging agents and actuators for ultrasound, magnetic resonance and several optical techniques. The diameter of GVs is a crucial parameter contributing to their mechanical stability, buoyancy function and evolution in host cells, as well as their properties in imaging applications. Despite its importance, reported diameters for the same types of GV differ depending on the method used for its assessment. Here, we provide an explanation for these discrepancies and utilize electron microscopy (EM) techniques to accurately estimate the diameter of the most commonly studied types of GVs. We show that during air drying on the EM grid, GVs flatten, leading to a ~1.5-fold increase in their apparent diameter. We demonstrate that GVs' diameter can be accurately determined by direct measurements from cryo-EM samples or alternatively indirectly derived from widths of flat collapsed and negatively stained GVs. Our findings help explain the inconsistency in previously reported data and provide accurate methods to measure GVs dimensions.
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Registered trials
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