Evidence map›Paper›PMID 33640990›Full record

ArticleArchives of microbiology2021

Epidemiology of molecular probes in Xpert MTB/RIF assay in Khyber Pakhtunkhwa, Pakistan.

Anwar Sheed Khan, Muhammad Tahir Khan, Sajid Ali, Taj Ali Khan, Muhammad Qasim, Arif Malik, Sajid Ali, Wasim Sajjad, Qurrat Ul Ain, Muhammad Irfan

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Article in Archives of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Anwar Sheed KhanProvincial Tuberculosis Reference, Laboratory Hayatabad Medical Complex, Peshawar, Pakistan.
Muhammad Tahir KhanInstitute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan. muhammad.tahir8@imbb.uol.edu.pk.
Sajid AliProvincial Tuberculosis Reference, Laboratory Hayatabad Medical Complex, Peshawar, Pakistan.
Taj Ali KhanDepartment of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan.
Muhammad QasimDepartment of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan.
Arif MalikInstitute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Sajid AliDepartment of Biotechnology, Abdul Wali Khan University, Mardan, Pakistan.
Wasim SajjadState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 7300000, China.
Qurrat Ul AinDepartment of Microbiology, Quaid-I- Azam University, Islamabad, Pakistan.
Muhammad IrfanCollege of Dentistry, Department of Oral Biology, University of Florida, Gainesville, FL, 32610, USA. irfanmuhammad@ufl.edu.ORCID http://orcid.org/0000-0002-1060-4436

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Regardless of a plethora of advanced diagnostics, TB and drug resistance remains a principal killer. We proposed gold nanoparticles (AuNPs) attached with probes to enhance the efficiency of GeneXpert MTB/RIF assay instead of conventional dye probes for molecular detection. A total of 15,000 samples were collected from TB suspects and subjected to Xpert MTB/RIF assay, where 6800 (45.3%) were detected as MTB positive, 280 (4.3%) were detected to harbor mutations in the RRDR, while invalid /errors were found in 690 (4.6%) cases. The mutations were detected by probe E, 199 (71.1%), while probes B and D, 30 and 26 (10% and 9%), respectively. In the Xpert MTB/RIF Assay were found mutations picked by probes E and B codons 529-533 (71%) and 512-518 (10%), respectively. The fast-rising works of TB nano-diagnostics, of Xpert probes, may improve by the applications of gold nanoparticle probes.

Indexed as

Antibiotics, AntitubercularCodonDrug Resistance, Multiple, BacterialGoldHumansMetal NanoparticlesMolecular ProbesMutationMycobacterium tuberculosisPakistanRifampinSensitivity and SpecificityTuberculosis, Multidrug-ResistantTuberculosis, PulmonaryAntibiotics, AntitubercularCodonGoldMolecular ProbesRifampinAuNPsProbeRRDRXpert MTB/RIF assay

Identifiers

PMID33640990

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.