ArticleJournal of experimental botany2021
Multigene editing reveals that MtCEP1/2/12 redundantly control lateral root and nodule number in Medicago truncatula.
Article in Journal of experimental botany, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plants.aBIOTECH · 2025Review
- Heterotrimeric G-protein subunits regulate plant architecture, pod development, seed size, and symbiotic nodulation inaBIOTECH · 2025Article
- A C-Terminally Encoded Peptide, MeCEP6, Promotes Nitrate Uptake in Cassava Roots.Plants (Basel, Switzerland) · 2025Article
- Decoding small peptides: Regulators of plant growth and stress resilience.Journal of integrative plant biology · 2025Review
- The Small Key to the Treasure Chest: Endogenous Plant Peptides Involved in Symbiotic Interactions.Plants (Basel, Switzerland) · 2025Review
- Peptide hormones in plants.Molecular horticulture · 2025Review
- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- CRISPR-Based Editing of thePlants (Basel, Switzerland) · 2024Article
- The S-acylation cycle of transcription factor MtNAC80 influences cold stress responses in Medicago truncatula.The Plant cell · 2024Article
- CRISPR/Cas9-Mediated Generation of Mutant Lines inBiology · 2024Article
- CEP hormones at the nexus of nutrient acquisition and allocation, root development, and plant-microbe interactions.Journal of experimental botany · 2024Review
- Article
- Review
- The thioesterase APT1 is a bidirectional-adjustment redox sensor.Nature communications · 2023Article
- The Medicago SymCEP7 hormone increases nodule number via shoots without compromising lateral root number.Plant physiology · 2023Article
- The small peptide CEP1 and the NIN-like protein NLP1 regulate NRT2.1 to mediate root nodule formation across nitrate concentrations.The Plant cell · 2023Article
- Plants Recruit Peptides and Micro RNAs to Regulate Nutrient Acquisition from Soil and Symbiosis.Plants (Basel, Switzerland) · 2023Review
- Identification and Expression Analysis of theInternational journal of molecular sciences · 2022Article
- Construction of genic male sterility system by CRISPR/Cas9 editing from model legume to alfalfa.Plant biotechnology journal · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multimember CEP (C-terminally Encoded Peptide) gene family is a complex group that is involved in various physiological activities in plants. Previous studies demonstrated that MtCEP1 and MtCEP7 control lateral root formation or nodulation, but these studies were based only on gain of function or artificial miRNA (amiRNA)/RNAi approaches, never knockout mutants. Moreover, an efficient multigene editing toolkit is not currently available for Medicago truncatula. Our quantitative reverse transcription-PCR data showed that MtCEP1, 2, 4, 5, 6, 7, 8, 9, 12, and 13 were up-regulated under nitrogen starvation conditions and that MtCEP1, 2, 7, 9, and 12 were induced by rhizobial inoculation. Treatment with synthetic MtCEP peptides of MtCEP1, 2, 4, 5, 6, 8, and 12 repressed lateral root emergence and promoted nodulation in the R108 wild type but not in the cra2 mutant. We optimized CRISPR/Cas9 [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9] genome editing system for M. truncatula, and thus created single mutants of MtCEP1, 2, 4, 6, and 12 and the double mutants Mtcep1/2C and Mtcep5/8C; however, these mutants did not exhibit significant differences from R108. Furthermore, a triple mutant Mtcep1/2/12C and a quintuple mutant Mtcep1/2/5/8/12C were generated and exhibited more lateral roots and fewer nodules than R108. Overall, MtCEP1, 2, and 12 were confirmed to be redundantly important in the control of lateral root number and nodulation. Moreover, the CRISPR/Cas9-based multigene editing protocol provides an additional tool for research on the model legume M. truncatula, which is highly efficient at multigene mutant generation.
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