An electrochemical method for detecting the biomarker 4-HPA by allosteric activation of Acinetobacterbaumannii reductase C1 subunit.

Somjai Teanphonkrang, Wipa Suginta, Jeerus Sucharitakul, Tamo Fukamizo, Pimchai Chaiyen, Albert Schulte

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Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

0.1field-weighted citation impact, top 60% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Somjai TeanphonkrangSchool of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand; School of Chemistry, Institute of Science, The Suranaree University of Technology (SUT), Nakhon Ratchasima, Thailand.

Wipa SugintaSchool of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand.

Jeerus SucharitakulDepartment of Biochemistry and Research Unit in Integrative Immuno-Microbial Biochemistry and Bioresponsive Nanomaterials, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

Tamo FukamizoDepartment of Advanced Bioscience, Kindai University, Nara, Japan.

Pimchai ChaiyenSchool of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand.

Albert SchulteSchool of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand; School of Chemistry, Institute of Science, The Suranaree University of Technology (SUT), Nakhon Ratchasima, Thailand. Electronic address: albert.s@vistec.ac.th.

Vidyasirimedhi Institute of Science and Technology · THChulalongkorn University · THKindai University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The C1 (reductase) subunit of 4-hydroxy-phenylacetate (4-HPA) 3-hydroxylase (HPAH) from the soil-based bacterium Acinetobacterbaumannii catalyzes NADH oxidation by molecular oxygen, with hydrogen peroxide as a by-product. 4-HPA is a potent allosteric modulator of C1, but also a known urinary biomarker for intestinal bacterial imbalance and for some cancers and brain defects. We thus envisioned that C1 could be used to facilitate 4-HPA detection. The proposed test protocol is simple and in situ and involves addition of NADH to C1 in solution, with or without 4-HPA, and direct acquisition of the H

Indexed as

Acinetobacter baumanniiAllosteric RegulationBiomarkersBiosensing TechniquesCatalysisElectrochemical TechniquesElectrodesHumansHydrogen PeroxideMixed Function OxygenasesNADOxidation-ReductionOxidoreductasesPhenylacetates3-hydroxyphenylacetate 6-hydroxylase4-hydroxyphenylacetateBiomarkersHydrogen PeroxideMixed Function OxygenasesNADOxidoreductasesPhenylacetatesphenylacetic acidallosteric regulationamperometrybiosensordisease biomarkerelectroanalysishydroxylaseoxidaseredox signalingreduced nicotinamide adenine dinucleotide (NADH)reductase

Identifiers

PMID33639166

DOI10.1016/j.jbc.2021.100467

PMCPMC8027283

OpenAlexW3129680047

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Textmetadata

LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.