ArticleMicrobial biotechnology2021
Accessing previously uncultured marine microbial resources by a combination of alternative cultivation methods.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Combating Antibacterial Resistance: The Integrative Role of Artificial Intelligence in Bio-Based Product Development.Antibiotics (Basel, Switzerland) · 2026Review
- A diffusion-based integrative approach for culturing previously uncultured bacteria from marine sediments.Marine life science & technology · 2025Article
- Harnessing iChip technology for novel antibiotic discovery from peat soil microbiomes to combat antimicrobial resistance.Frontiers in microbiology · 2025Article
- Impact of growth conditions on the abundance and diversity of cultivable bacteria recovered fromFEMS microbes · 2025Article
- Cultivation of Diverse Novel Marine Bacteria from Deep Ocean Sediment Using Spent Culture Supernatant of Ca. Bathyarchaeia Enrichment.Journal of microbiology (Seoul, Korea) · 2024Article
- Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability.Microbiology spectrum · 2023Article
- Modern Trends in Natural Antibiotic Discovery.Life (Basel, Switzerland) · 2023Review
- Shedding light on the composition of extreme microbial dark matter: alternative approaches for culturing extremophiles.Frontiers in microbiology · 2023Review
- A targeted liquid cultivation method for previously uncultured non-colony forming microbes.Frontiers in microbiology · 2023Article
- Marine Bioprospecting, Biocatalysis and Process Development.Microorganisms · 2022Review
- Review
- Cultivating marine bacteria under laboratory conditions: Overcoming the "unculturable" dogma.Frontiers in bioengineering and biotechnology · 2022Article
- Article
- Accessing previously uncultured marine microbial resources by a combination of alternative cultivation methods.Microbial biotechnology · 2021Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Few microbes can grow under laboratory conditions, highlighting the fact that the majority of microbes in environment are still uncultured and untapped resources. This study used alternative cultivation methods, diffusion chambers (DC), dilution-to-extinction culture (DTE) and modified agar preparation step (PS media) to cultivate previously uncultured marine bacterial species. These methods were applied to samples from a coastal intertidal zone, and the results were compared with those from standard direct plating (SDP) cultivation. Among the strains isolated with DC, DTE and PS media methods, 28%, 48% and 33% were novel species, respectively, while the SDP method resulted in the isolation of only 9% of novel species. Most isolates were unique to the method used for their cultivation. This implies that each method is selective in its own way, which is different from SDP, thus able to access species that are difficult to obtain using conventional approaches. Comparing the diversity showed that 75 genera were recovered by the alternative methods, 2.7 times higher than that of the SDP cultivation, which constituted 45% of total diversity from culture-independent sequencing. We conclude that combining alternative cultivation methods represents a highly promising key for accessing 'microbial dark matter'.
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