ArticlePlant biotechnology journal2021
Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 90 citations in OpenAlex.
- Asparagine Synthetase 1 regulates rice seminal root elongation via jasmonic acid metabolism.Plant cell reports · 2026Article
- Field Trials and Baking Studies of Ultra-Low Asparagine, Genome Edited (CRISPR/Cas9) and Mutant (TILLING) Wheat.Plant biotechnology journal · 2026Article
- Agronomic, genetic and biotechnological approaches to lower free asparagine content in cereals: state of the art and perspectives.Food chemistry: X · 2026Review
- Disruption of Asparagine Synthetase Is Associated to Increased Biomass in Lotus japonicus.Plant biotechnology journal · 2026Article
- CRISPR/Cas Genome Editing and Its Applications in Cereal Crop Improvement.Plant-environment interactions (Hoboken, N.J.) · 2026Review
- The Multifaceted Functions of Plant Asparagine Synthetase: Regulatory Mechanisms and Functional Diversity in Growth and Defense.Plants (Basel, Switzerland) · 2026Review
- The Potential of NGTs to Overcome Constraints in Plant Breeding and Their Regulatory Implications.International journal of molecular sciences · 2025Review
- Exploring Molecular Mechanisms in Wheat against Drought Stress from Sustainable Resilience Strategies To Emerging Innovative Approaches.Current microbiology · 2025Review
- From convention to innovation: the role of genetic modification and genome editing in Australian wheat breeding.AoB PLANTS · 2025Review
- CRISPR/Cas9-mediated mutation of GhCAD decreases the gossypol content of cottonseed.Journal of biological engineering · 2025Article
- Asparagine Synthetase GenePlants (Basel, Switzerland) · 2025Article
- Advances in genome editing in plants within an evolving regulatory landscape, with a focus on its application in wheat breeding.Journal of plant biochemistry and biotechnology · 2025Review
- Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat.Frontiers in genome editing · 2025Article
- The Potential of CRISPR/Cas9 to Circumvent the Risk Factor NeurotoxinCurrent issues in molecular biology · 2024Review
- Low asparagine wheat: Europe's first field trial of genome edited wheat amid rapidly changing regulations on acrylamide in food and genome editing of crops.Breeding science · 2024Article
- Enhancement of specialized metabolites using CRISPR/Cas gene editing technology in medicinal plants.Frontiers in plant science · 2024Review
- Genetic control of grain amino acid composition in a UK soft wheat mapping population.The plant genome · 2023Article
- Reducing the Risk of Acrylamide and Other Processing Contaminant Formation in Wheat Products.Foods (Basel, Switzerland) · 2023Review
- CRISPR/Cas9 genome editing in wheat: enhancing quality and productivity for global food security-a review.Functional & integrative genomics · 2023Review
- Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
Free asparagine is the precursor for acrylamide, which forms during the baking, toasting and high-temperature processing of foods made from wheat. In this study, CRISPR/Cas9 was used to knock out the asparagine synthetase gene, TaASN2, of wheat (Triticum aestivum) cv. Cadenza. A 4-gRNA polycistronic gene was introduced into wheat embryos by particle bombardment and plants were regenerated. T1 plants derived from 11 of 14 T0 plants were shown to carry edits. Most edits were deletions (up to 173 base pairs), but there were also some single base pair insertions and substitutions. Editing continued beyond the T1 generation. Free asparagine concentrations in the grain of plants carrying edits in all six TaASN2 alleles (both alleles in each genome) were substantially reduced compared with wildtype, with one plant showing a more than 90 % reduction in the T2 seeds. A plant containing edits only in the A genome alleles showed a smaller reduction in free asparagine concentration in the grain, but the concentration was still lower than in wildtype. Free asparagine concentration in the edited plants was also reduced as a proportion of the free amino acid pool. Free asparagine concentration in the T3 seeds remained substantially lower in the edited lines than wildtype, although it was higher than in the T2 seeds, possibly due to stress. In contrast, the concentrations of free glutamine, glutamate and aspartate were all higher in the edited lines than wildtype. Low asparagine seeds showed poor germination but this could be overcome by exogenous application of asparagine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.