ArticleCommunications biology2021
A versatile microfluidic platform measures hyphal interactions between Fusarium graminearum and Clonostachys rosea in real-time.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Self-grown mycelium in confined geometries as nanofluidic devices.Nature communications · 2026Article
- Differences in mycelial turnover and persistence of wood-decay fungi at the microscale.The New phytologist · 2026Article
- Methods for evaluating bacterial dispersal on hyphal networks.microLife · 2026Article
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- Hierarchical Structure of the Program Used by Filamentous Fungi to Navigate in Confining Microenvironments.Biomimetics (Basel, Switzerland) · 2025Article
- Electrical signaling in fungi: past and present challenges.FEMS microbiology reviews · 2025Review
- Measurement of the effectiveness of Clonostachys rosea in reducing Fusarium biomass on wheat straw.Journal of applied genetics · 2024Article
- Genotypic variation in winter wheat for fusarium foot rot and its biocontrol using Clonostachys rosea.G3 (Bethesda, Md.) · 2024Article
- Visualizing liquid distribution across hyphal networks with cellular resolution.Biomicrofluidics · 2024Article
- Recent Advances in Microfluidics for the Early Detection of Plant Diseases in Vegetables, Fruits, and Grains Caused by Bacteria, Fungi, and Viruses.Journal of agricultural and food chemistry · 2024Review
- Article
- Fabricated devices for performing bacterial-fungal interaction experiments across scales.Frontiers in microbiology · 2024Review
- Containment ofJournal of fungi (Basel, Switzerland) · 2023Article
- Microbial-Based Products to Control Soil-Borne Pathogens: Methods to Improve Efficacy and to Assess Impacts on Microbiome.Microorganisms · 2023Review
- Fungal drops: a novel approach for macro- and microscopic analyses of fungal mycelial growth.microLife · 2023Article
- Spatial scales of competition and a growth-motility trade-off interact to determine bacterial coexistence.Royal Society open science · 2022Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- A versatile microfluidic platform measures hyphal interactions between Fusarium graminearum and Clonostachys rosea in real-time.Communications biology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Routinely, fungal-fungal interactions (FFI) are studied on agar surfaces. However, this format restricts high-resolution dynamic imaging. To gain experimental access to FFI at the hyphal level in real-time, we developed a microfluidic platform, a FFI device. This device utilises microchannel geometry to enhance the visibility of hyphal growth and provides control channels to allow comparisons between localised and systemic effects. We demonstrate its function by investigating the FFI between the biological control agent (BCA) Clonostachys rosea and the plant pathogen Fusarium graminearum. Microscope image analyses confirm the inhibitory effect of the necrotrophic BCA and we show that a loss of fluorescence in parasitised hyphae of GFP-tagged F. graminearum coincides with the detection of GFP in mycelium of C. rosea. The versatility of our device to operate under both water-saturated and nutrient-rich as well as dry and nutrient-deficient conditions, coupled with its spatio-temporal output, opens new opportunities to study relationships between fungi.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.