ArticleNature communications2021
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Rat cell-derived kidney generation via interspecies blastocyst complementation in an Osr1-KO mouse model.Stem cell reports · 2026Article
- African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation.Cell reports methods · 2026Article
- Considerations for recipient male selection in a livestock Surrogate Sire breeding strategy.The Journal of reproduction and development · 2026Review
- Transcription factor-mediated germ cell induction in rats reveals ETV4 cooperates with germline specifiers.Stem cell reports · 2025Article
- Generation of parathyroid glands from pluripotent stem cells.Endocrine journal · 2025Review
- Morula complementation restores male germline inPNAS nexus · 2025Article
- Blastocyst complementation: current progress and future directions in xenogeneic organogenesis.Stem cell research & therapy · 2025Review
- Blastocyst complementation-based rat-derived heart generation reveals cardiac anomaly barriers to interspecies chimera development.iScience · 2024Article
- In vivo fertilization improved the cryotolerance and developmental ability of vitrified-warmed rat fertilized oocytes.Scientific reports · 2024Article
- Parathyroid Gland Generation from Pluripotent Stem Cells.Endocrinology and metabolism (Seoul, Korea) · 2024Review
- Generating an organ-deficient animal model using a multi-targeted CRISPR-Cas9 system.Scientific reports · 2024Article
- Skin graft with dermis and appendages generated in vivo by cell competition.Nature communications · 2024Article
- Generation of rat forebrain tissues in mice.Cell · 2024Article
- Interspecies chimerism with human embryonic stem cells generates functional human dopamine neurons at low efficiency.Stem cell reports · 2024Article
- Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells.Cell reports methods · 2023Article
- Human and Pig Pluripotent Stem Cells: From Cellular Products to Organogenesis and Beyond.Cells · 2023Review
- Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Development of a Method for the In Vivo Generation of Allogeneic Hearts in Chimeric Mouse Embryos.International journal of molecular sciences · 2023Article
- Germline ablation achieved via CRISPR/Cas9 targeting ofFrontiers in genome editing · 2023Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Murine animal models from genetically modified pluripotent stem cells (PSCs) are essential for functional genomics and biomedical research, which require germline transmission for the establishment of colonies. However, the quality of PSCs, and donor-host cell competition in chimeras often present strong barriers for germline transmission. Here, we report efficient germline transmission of recalcitrant PSCs via blastocyst complementation, a method to compensate for missing tissues or organs in genetically modified animals via blastocyst injection of PSCs. We show that blastocysts from germline-deficient Prdm14 knockout rats provide a niche for the development of gametes originating entirely from the donor PSCs without any detriment to somatic development. We demonstrate the potential of this approach by creating PSC-derived Pax2/Pax8 double mutant anephric rats, and rescuing germline transmission of a PSC carrying a mouse artificial chromosome. Furthermore, we generate mouse PSC-derived functional spermatids in rats, which provides a proof-of-principle for the generation of xenogenic gametes in vivo. We believe this approach will become a useful system for generating PSC-derived germ cells in the future.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.