ArticleNature communications2021
Optical tweezers-controlled hotspot for sensitive and reproducible surface-enhanced Raman spectroscopy characterization of native protein structures.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- A Review of SERS-Based Bacterial Detection from Nanomaterials to Integrated Clinical Platforms.Biosensors · 2026Review
- Plasmonic Optical Tweezers and Surface-Enhanced Raman Spectroscopy: Fundamentals, Single-Entity Applications, and the Evolving Role of Artificial Intelligence.Bioengineering (Basel, Switzerland) · 2026Review
- Next-Generation SERS Probes: Engineering Hotspots, Intelligent Molecular Targeting, and AI-Driven Spectral Analysis for Emerging Applications.Nanomaterials (Basel, Switzerland) · 2026Review
- Bioanalysis Using Surface-Enhanced Raman Spectroscopy: From the Perspective of Single Molecules.ACS nano · 2026Review
- SERS-Based Nano- and Microsystems Toward Biomedical Applications.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Spatiotemporal evaluation of antithrombotic performance for biomaterials through monitoring β-sheet conformation changes at the blood interface.Bioactive materials · 2026Article
- Recent advances in nanomaterial-based optical biosensors and their biomedical and biopharmaceutical applications.Journal of pharmaceutical analysis · 2026Review
- Se(IV) mediated anisotropic etching of bare gold nanorods for mutant biomarker sensing by surface-enhanced electrochemiluminescence.Mikrochimica acta · 2025Article
- Revolutionizing healthcare with nanosensor technology.RSC advances · 2025Review
- Advances in Multifunctional Nanoagents and SERS-Based Multimodal Sensing for Biotoxin in Foods.Foods (Basel, Switzerland) · 2025Review
- Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges.ACS applied materials & interfaces · 2025Review
- SERS-Active Micro/Nanomachines for Biosensing.Biosensors · 2025Review
- Advancements in SERS: Revolutionizing Biomedical Analysis and Applications.Nanotheranostics · 2025Review
- Thermo-optical tweezers based on photothermal waveguides.Microsystems & nanoengineering · 2024Article
- Accurate categorization and rapid pathological diagnosis correction with Micro-Raman technique in human lung adenocarcinoma infiltration level.Translational lung cancer research · 2024Article
- Rapidly determining the 3D structure of proteins by surface-enhanced Raman spectroscopy.Science advances · 2023Article
- Efficient optical plasmonic tweezer-controlled single-molecule SERS characterization of pH-dependent amylin species in aqueous milieus.Nature communications · 2023Article
- Switching on Versatility: Recent Advances in Switchable Plasmonic Nanostructures.Small science · 2023Article
- Einstein Model of a Graph to Characterize Protein Folded/Unfolded States.Molecules (Basel, Switzerland) · 2023Article
- Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful tool to detect biomolecules in aqueous environments. However, it is challenging to identify protein structures at low concentrations, especially for the proteins existing in an equilibrium mixture of various conformations. Here, we develop an in situ optical tweezers-coupled Raman spectroscopy to visualize and control the hotspot between two Ag nanoparticle-coated silica beads, generating tunable and reproducible SERS enhancements with single-molecule level sensitivity. This dynamic SERS detection window is placed in a microfluidic flow chamber to detect the passing-by proteins, which precisely characterizes the structures of three globular proteins without perturbation to their native states. Moreover, it directly identifies the structural features of the transient species of alpha-synuclein among its predominant monomers at physiological concentration of 1 μM by reducing the ensemble averaging. Hence, this SERS platform holds the promise to resolve the structural details of dynamic, heterogeneous, and complex biological systems.
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