Evidence map›Paper›PMID 33636109›Full record

ArticleThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

Innovative approaches to improve serodiagnosis of Strongyloides stercoralis infection.

Marcelo Arantes Levenhagen, Patrícia Tiemi Fujimura, Fabiana de Almeida Araújo Santos, Lara Vecchi, Renata Pereira Alves-Balvedi, Ana Graci Brito Madurro, João Marcos Madurro, Julia Maria Costa-Cruz, Luiz Ricardo Goulart

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Marcelo Arantes LevenhagenUniversidade Federal de Uberlândia, Instituto de Ciências Biomédicas, Laboratório de Diagnóstico de Parasitoses, Uberlândia, MG, Brazil. Electronic address: marcelolevenhagen@yahoo.com.br.
Patrícia Tiemi FujimuraUniversidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Laboratório de Nanobiotecnologia, Uberlândia, MG, Brazil.
Fabiana de Almeida Araújo SantosUniversidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Laboratório de Nanobiotecnologia, Uberlândia, MG, Brazil.
Lara VecchiUniversidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Laboratório de Nanobiotecnologia, Uberlândia, MG, Brazil.
Renata Pereira Alves-BalvediUniversidade Federal de Uberlândia, Laboratório de Filmes Poliméricos e Nanotecnologia, Instituto de Química, Uberlândia, MG, Brazil.
Ana Graci Brito MadurroUniversidade Federal de Uberlândia, Laboratório de Filmes Poliméricos e Nanotecnologia, Instituto de Química, Uberlândia, MG, Brazil.
João Marcos MadurroUniversidade Federal de Uberlândia, Laboratório de Filmes Poliméricos e Nanotecnologia, Instituto de Química, Uberlândia, MG, Brazil.
Julia Maria Costa-CruzUniversidade Federal de Uberlândia, Instituto de Ciências Biomédicas, Laboratório de Diagnóstico de Parasitoses, Uberlândia, MG, Brazil.
Luiz Ricardo GoulartUniversidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Laboratório de Nanobiotecnologia, Uberlândia, MG, Brazil; Department of Medical Microbiology and Immunology, University of California, Davis, CA, USA.
Universidade Federal de Uberlândia · BRUniversity of California Davis Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Strongyloidiasis is a helminthiasis of neglected condition that has no gold standard parasitological diagnosis due to the intermittent release of larvae in feces. This study aimed to use an scFv (single chain variable fragment) obtained by Phage Display, previously validated to detect immune complexes in serum samples from individuals infected with Strongyloides stercoralis by enzyme-linked immunosorbent assay (ELISA). Now the ability of scFv to detect the immune complexes was verified by immunofluorescence, flow cytometry using magnetic beads and surface plasmon resonance (SPR). As ELISA, the SPR, immunofluorescence and flow cytometry demonstrated the ability of scFv to detect immune complexes in sera from individuals with strongyloidiasis and discriminate them from sera of individuals with other parasitic diseases and healthy individuals. Besides de conventional ELISA, the novel approaches can also be promptly applied as auxiliary diagnostic tools to the existing parasitological method for accurate diagnosis of human strongyloidiasis.

Indexed as

Strongyloides stercoralisStrongyloidiasisAnimalsAntibodies, HelminthEnzyme-Linked Immunosorbent AssayFecesHumansImmunoglobulin GSerologic TestsAntibodies, HelminthImmunoglobulin GDetection methodsHuman strongyloidiasisImmune complexesscFvStrongyloides stercoralis

Identifiers

PMID33636109
PMCPMC9392097
OpenAlexW3132930350

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.