ArticleNature methods2021
Photoblueing of organic dyes can cause artifacts in super-resolution microscopy.
Article in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Subcellular photochemistry for precision spatial protein targeting.Nature reviews. Chemistry · 2026Review
- Spontaneous and indefinite blinking in upconverting nanoparticles for ångström-precision multicolour super-resolution imaging.Nature nanotechnology · 2026Article
- Super-resolution atlas of SARS-CoV-2 infection reveals protease-dependent organelle maturation, dsRNA landscapes, and intracellular structural proteins.Nature communications · 2026Article
- Photochemical modification of two fluorene-based molecules with DNA intercalating and anti-methicillin resistant Staphylococcus aureus activity.The Journal of biological chemistry · 2026Article
- Single-Molecule Fluorescence Defines a New Dye Chemistry.Chemical & biomedical imaging · 2026Review
- Photophysics of Organic Fluorophore Photobluing and Its Applications in Fluorescence and Super-Resolution Microscopy.Accounts of chemical research · 2026Article
- Cyanines Substituted on the Polymethine Chain: Synthesis, Resulting Properties, and Application Use Cases.ChemPlusChem · 2025Review
- Time-Resolved Fluorescence Detection of Nicked DNA via Site-Specific Stacking of Sulfo-Cy3: The Role of Charge, Polarity, Linker, and Sequence Context.The journal of physical chemistry. B · 2025Article
- Tailoring Tubular Supramolecular Polymers with Polar Lumens to Host Hydrophilic Dye Molecules.Angewandte Chemie (International ed. in English) · 2025Article
- Highly accurate image registration for 3D multiplexed cyclic imaging using dense labeling in expandable tissue gels.PLoS biology · 2025Article
- Bridge to a brighter future in fluorescence imaging.Nature methods · 2025Article
- A palette of bridged bicycle-strengthened fluorophores.Nature methods · 2025Article
- Super-photostable organic dye for long-term live-cell single-protein imaging.Nature methods · 2025Article
- Indocarbocyanine-Indodicarbocyanine (sCy3-sCy5) Absorptive Interactions in Conjugates and DNA Duplexes.Molecules (Basel, Switzerland) · 2024Article
- Strongly coupled plasmon-exciton polaritons for photobleaching suppression.Nanophotonics (Berlin, Germany) · 2024Article
- Multiplexed Nanoscopy via Buffer Exchange.ACS nano · 2024Article
- Mechanistic Insight into the Thermal "Blueing" of Cyanine Dyes.Journal of the American Chemical Society · 2024Article
- Thermal Truncation of Heptamethine Cyanine Dyes.Journal of the American Chemical Society · 2024Article
- Effects and avoidance of photoconversion-induced artifacts in confocal and STED microscopy.Nature methods · 2024Article
- Phase-shifting optothermal microscopy enables live-cell mid-infrared hyperspectral imaging of large cell populations at high confluency.Science advances · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging. Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented.
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