ArticleScientific reports2021
Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 43 citations in OpenAlex.
- Uncovering mitotic ultrastructure in the native hair follicle using volume electron microscopy.Journal of cell science · 2026Article
- Correcting Non-Uniform Milling in FIB-SEM Images with Unsupervised Cross-Plane Image-to-Image Translation.bioRxiv : the preprint server for biology · 2025Article
- Article
- Olfaction with legs-Spiders use wall-pore sensilla for pheromone detection.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Organ-resolved lipid mapping inFrontiers in chemistry · 2025Article
- WASPSYN: A Challenge for Domain Adaptive Synapse Detection in Microwasp Brain Connectomes.IEEE transactions on medical imaging · 2024Article
- Minimal resin embedding of SBF-SEM samples reduces charging and facilitates finding a surface-linked region of interest.Frontiers in zoology · 2023Article
- The 3D ultrastructure of the chordotonal organs in the antenna of a microwasp remains complex although simplified.Scientific reports · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Revision of the World Species ofInsects · 2022Article
- Field-Emission Scanning Electron Microscope as a Tool for Large-Area and Large-Volume Ultrastructural Studies.Animals : an open access journal from MDPI · 2021Review
- High-precision targeting workflow for volume electron microscopy.The Journal of cell biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with sample preparation, since the methods work poorly with heterogeneous (consisting of tissues different in structure and in chemical composition) samples and require expensive equipment and usually much time. We have developed a simple protocol allows preparing heterogeneous biological samples suitable for 3D-EM in a laboratory that has a standard supply of equipment and reagents for electron microscopy. This protocol, combined with focused ion-beam scanning electron microscopy, makes it possible to study 3D ultrastructure of complex biological samples, e.g., whole insect heads, over their entire volume at the cellular and subcellular levels. The protocol provides new opportunities for many areas of study, including connectomics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.