ArticleScientific reports2021
Massively parallel sequencing of 25 autosomal STRs including SE33 in four population groups for forensic applications.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 31 citations in OpenAlex.
- Large-scale human genomic resources drive innovations toward forensic intelligent source attribution and precision identification.International journal of legal medicine · 2026Review
- Swedish population data for 37 autosomal, 12 X-chromosomal and 23 Y-chromosomal STR loci.International journal of legal medicine · 2026Article
- Beyond the Core: The Role of Supplementary Short Tandem Repeats in Forensic Genetics.International journal of molecular sciences · 2026Review
- Revisiting guidance on population sampling for highly polymorphic STR loci.Forensic science international. Genetics · 2026Article
- Evaluation of gain in statistical power for kinship analysis using sequence-based versus length-based STR genotyping.International journal of legal medicine · 2026Article
- Characterization of a novel unliked 12 X-STR typing assay for forensic purposes in an admixed Rio de Janeiro population sample.Genetics and molecular biology · 2025Article
- STRategy: A support system for collecting and analyzing next-generation sequencing data of short tandem repeats for forensic science.PloS one · 2023Article
- Sequence Variations of 31 Υ-Chromosomal Short Tandem Repeats Analyzed by Massively Parallel Sequencing in Three U.S. Population Groups and Korean Population.Journal of Korean medical science · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The introduction of massively parallel sequencing (MPS) in forensic investigation enables sequence-based large-scale multiplexing beyond size-based analysis using capillary electrophoresis (CE). For the practical application of MPS to forensic casework, many population studies have provided sequence data for autosomal short tandem repeats (STRs). However, SE33, a highly polymorphic STR marker, has little sequence-based data because of difficulties in analysis. In this study, 25 autosomal STRs were analyzed, including SE33, using an in-house MPS panel for 350 samples from four populations (African-American, Caucasian, Hispanic, and Korean). The barcoded MPS library was generated using a two-step PCR method and sequenced using a MiSeq System. As a result, 99.88% genotype concordance was obtained between length- and sequence-based analyses. In SE33, the most discordances (eight samples, 0.08%) were observed because of the 4 bp deletion between the CE and MPS primer binding sites. Compared with the length-based CE method, the number of alleles increased from 332 to 725 (2.18-fold) for 25 autosomal STRs in the sequence-based MPS method. Notably, additional 129 unique alleles, a 4.15-fold increase, were detected in SE33 by identifying sequence variations. This population data set provides sequence variations and sequence-based allele frequencies for 25 autosomal STRs.
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