ArticleBMC biology2021
20E-mediated regulation of BmKr-h1 by BmKRP promotes oocyte maturation.
Article in BMC biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
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- Convergent evolution of a conserved molecular network underlies parenting and sociality.Nature reviews. Genetics · 2026Review
- Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis.Nature communications · 2025Article
- 20E-inducedProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Novel regulation pathway of eclosion hormones in Tribolium castaneum by distinct transcription factors through the initiation of 20-hydroxyecdysone.The Journal of biological chemistry · 2024Article
- Cyp6g2 is the major P450 epoxidase responsible for juvenile hormone biosynthesis in Drosophila melanogaster.BMC biology · 2024Article
- Ecdysone-controlled nuclear receptor ERR regulates metabolic homeostasis in the disease vector mosquito Aedes aegypti.PLoS genetics · 2024Article
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- Mechanism of hyperproteinemia-induced damage to female reproduction in a genetic silkworm model.iScience · 2023Article
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- IPS (In-Plant System) Delivery of Double-StrandedInternational journal of molecular sciences · 2023Article
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- Crosstalk between Nutrition, Insulin, Juvenile Hormone, and Ecdysteroid Signaling in the Classical Insect Model,International journal of molecular sciences · 2022Article
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- Cyromazine Effects the Reproduction ofInsects · 2022Article
- Review
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Authors and funding
9 authors at 2 institutions in 2 countries.
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Abstract
backgroundKrüppel homolog 1 (Kr-h1) is a critical transcription factor for juvenile hormone (JH) signaling, known to play a key role in regulating metamorphosis and adult reproduction in insects. Kr-h1 can also be induced by molting hormone 20-hydroxyecdysone (20E), however, the underlying mechanism of 20E-induced Kr-h1 expression remains unclear. In the present study, we investigated the molecular mechanism of Kr-h1 induction by 20E in the reproductive system of a model lepidopteran insect, Bombyx mori.
resultsDevelopmental and tissue-specific expression analysis revealed that BmKr-h1 was highly expressed in ovaries during the late pupal and adult stages and the expression was induced by 20E. RNA interference (RNAi)-mediated depletion of BmKr-h1 in female pupae severely repressed the transcription of vitellogenin receptor (VgR), resulting in the reduction in vitellogenin (Vg) deposition in oocytes. BmKr-h1 specifically bound the Kr-h1 binding site (KBS) between - 2818 and - 2805 nt upstream of BmVgR and enhanced the transcription of BmVgR. A 20E cis-regulatory element (CRE) was identified in the promoter of BmKr-h1 and functionally verified using luciferase reporter assay, EMSA and DNA-ChIP. Using pull-down assays, we identified a novel transcription factor B. mori Kr-h1 regulatory protein (BmKRP) that specifically bound the BmKr-h1 CRE and activated its transcription. CRISPR/Cas9-mediated knockout of BmKRP in female pupae suppressed the transcription of BmKr-h1 and BmVgR, resulting in arrested oogenesis.
conclusionWe identified BmKRP as a new transcription factor mediating 20E regulation of B. mori oogenesis. Our data suggests that induction of BmKRP by 20E regulates BmKr-h1 expression, which in turn induces BmVgR expression to facilitate Vg uptake and oogenesis.
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