ArticlePlant physiology2021
PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 17 citations in OpenAlex.
- The PHO1 protein family in phosphate export and beyond.Plant physiology · 2026Review
- Telomere-to-telomere genome of Stylosanthes guianensis uncovers symbiotic adaptation to phosphorus-deficient soils.Genome biology · 2026Article
- The SPX protein family in plants: from phosphate sensors to multifunctional signaling hubs.Stress biology · 2026Review
- Medicago phosphate exporter PHO1.3 regulates arbuscular mycorrhizal symbiosis.BMC plant biology · 2026Article
- Molecular mechanisms modulating beneficial plant root-microbe interactions: What's common?Plant communications · 2026Review
- Genome-wide identification and analysis of phosphate utilization related genes (PURs) reveal their roles involved in low phosphate responses in Brassica napus L.BMC plant biology · 2025Article
- Nutrient-dependent regulation of symbiotic nitrogen fixation in legumes.Horticulture research · 2025Article
- Structural mechanism underlying PHO1;H1-mediated phosphate transport in Arabidopsis.Nature plants · 2025Article
- Milestones in understanding transport, sensing, and signaling of the plant nutrient phosphorus.The Plant cell · 2024Review
- Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis.Nature plants · 2024Article
- Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago.Nature plants · 2023Article
- Article
- Improving phosphorus acquisition efficiency through modification of root growth responses to phosphate starvation in legumes.Frontiers in plant science · 2023Review
- Roles of plastid-located phosphate transporters in carotenoid accumulation.Frontiers in plant science · 2022Review
- Argonaute5 and its associated small RNAs modulate the transcriptional response during the rhizobia-Frontiers in plant science · 2022Article
- Intracellular phosphate sensing and regulation of phosphate transport systems in plants.Plant physiology · 2021Review
- PHO1 proteins mediate phosphate transport in the legume-rhizobium symbiosis.Plant physiology · 2021Article
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legumes play an important role in the soil nitrogen availability via symbiotic nitrogen fixation (SNF). Phosphate (Pi) deficiency severely impacts SNF because of the high Pi requirement of symbiosis. Whereas PHT1 transporters are involved in Pi uptake into nodules, it is unknown how Pi is transferred from the plant infected cells to nitrogen-fixing bacteroids. We hypothesized that Medicago truncatula genes homologous to Arabidopsis PHO1, encoding a vascular apoplastic Pi exporter, are involved in Pi transfer to bacteroids. Among the seven MtPHO1 genes present in M. truncatula, we found that two genes, namely MtPHO1.1 and MtPHO1.2, were broadly expressed across the various nodule zones in addition to the root vascular system. Expressions of MtPHO1.1 and MtPHO1.2 in Nicotiana benthamiana mediated specific Pi export. Plants with nodule-specific downregulation of both MtPHO1.1 and MtPHO1.2 were generated by RNA interference (RNAi) to examine their roles in nodule Pi homeostasis. Nodules of RNAi plants had lower Pi content and a three-fold reduction in SNF, resulting in reduced shoot growth. Whereas the rate of 33Pi uptake into nodules of RNAi plants was similar to control, transfer of 33Pi from nodule cells into bacteroids was reduced and bacteroids activated their Pi-deficiency response. Our results implicate plant MtPHO1 genes in bacteroid Pi homeostasis and SNF via the transfer of Pi from nodule infected cells to bacteroids.
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