Evidence map›Paper›PMID 33630165›Full record

ArticleClinical oral investigations2021

Regeneration of pulp-dentin complex using human stem cells of the apical papilla: in vivo interaction with two bioactive materials.

Diana B Sequeira, Ana Rafaela Oliveira, Catarina M Seabra, Paulo J Palma, Carlos Ramos, Maria H Figueiredo, Ana C Santos, Ana L Cardoso, João Peça, João Miguel Santos

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
9.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 62 citations in OpenAlex.

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  17. Influence of photobiomodulation therapy on regenerative potential of non-vital mature permanent teeth in healthy canine dogs.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Diana B SequeiraCNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Ana Rafaela OliveiraCNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Catarina M SeabraCNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Paulo J PalmaInstitute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
Carlos RamosCoimbra Institute for Clinical and Biomedical Research (iCBR) - CIBB, University of Coimbra, Coimbra, Portugal.
Maria H FigueiredoInstitute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
Ana C SantosCoimbra Institute for Clinical and Biomedical Research (iCBR) - CIBB, University of Coimbra, Coimbra, Portugal.
Ana L CardosoCNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
João PeçaCNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
João Miguel SantosInstitute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal. jsantos@fmed.uc.pt.ORCID http://orcid.org/0000-0002-2865-9689
University of Coimbra · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo compare the regenerative properties of human stem cells of the apical papilla (SCAPs) embedded in a platelet-rich plasma (PRP) scaffold, when implanted in vivo using an organotypic model composed of human root segments, with or without the presence of the bioactive cements - ProRoot MTA or Biodentine. MATERIAL AND

methodsSCAPs were isolated from third molars with incomplete rhizogenesis and expanded and characterized in vitro using stem cell and surface markers. The pluripotency of these cells was also assessed using adipogenic, chondrogenic, and osteogenic differentiation protocols. SCAPs together with a scaffold of PRP were added to the root segment lumen and the organotypic model implanted on the dorsal region of immunodeficient rats for a period of 4 months.

resultsPresence of SCAPs induced de novo formation of dentin-like and pulp-like tissue. A barrier of either ProRoot MTA or Biodentine did not significantly affect the fraction of sections from roots segments observed to contain deposition of hard material (P > 0.05). However, the area of newly deposited dentin was significantly greater in segments containing a barrier of Biodentine compared with ProRoot MTA (P < 0.001). CONCLUSIONS AND CLINICAL RELEVANCE: SCAPs offer a viable alternative to other dental stem cells (DSCs) in their regenerative properties when enclosed in the microenvironment of human tooth roots. The present study also suggests that the presence of bioactive materials does not hinder or impede the formation of new hard tissues, but the presence of Biodentine may promote greater mineralized tissue deposition.

Indexed as

OsteogenesisStem CellsAnimalsCell DifferentiationCells, CulturedDental PapillaDental PulpDentinHumansRatsRegenerationCell differentiationEndodonticsRegenerationRegenerative endodonticsStem cellsStem cells from the apical papilla

Identifiers

PMID33630165
OpenAlexW3132621537

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.