ArticleeLife2021
A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed.
- Arabidopsis GOLDEN2-LIKE transcription factors interact with ABA-INSENSITIVE 4 to modulate cotyledon greening in light-exposed etiolated seedlings.Journal of experimental botany · 2026Article
- Turning over a green leaf: integrating light signals toward chloroplast establishment.Plant physiology · 2026Review
- The yellow-in-the-dark chIL Chlamydomonas mutant as a model for time-resolved chloroplast biogenesis and retrograde signaling.Plant physiology · 2026Article
- Inosine monophosphate dehydrogenases are key players for functional development in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Integrated multi-omics profiling reveals dynamic regulation of light-induced chloroplast biogenesis in Brassica napus seedlings.Nucleic acids research · 2026Article
- Understanding and modifying starch metabolism to limit yield losses in field-grown cassava.Plant physiology · 2026Article
- Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts.Nature communications · 2026Article
- Regulation of chloroplast biogenesis and differentiation.Journal of experimental botany · 2026Review
- Role of CIA2 and CIL in the Regulation of Chloroplast Development During Photomorphogenesis in Arabidopsis.Cells · 2026Article
- Effects of LED light treatments on the bioactive composition and functional properties of pea (Frontiers in plant science · 2026Article
- Article
- Plant MutS2 proteins function in plastid ribosome quality control.bioRxiv : the preprint server for biology · 2025Article
- Plastids in a Pinch: Coordinating Stress and Developmental Responses Through Retrograde Signalling.Plant, cell & environment · 2025Review
- Retrograde signals control dynamic changes to the chromatin state at photosynthesis-associated loci.Nature communications · 2025Article
- Shrink or expand? Just relax! Bidirectional grana structural dynamics as early light-induced regulator of photosynthesis.The New phytologist · 2025Article
- GRANA: An AI-based tool for accelerating chloroplast grana nanomorphology analysis using hybrid intelligence.Plant physiology · 2025Article
- MS-RT: A Method for Evaluating MS/MS Clustering Performance for Metabolomics Data.Journal of proteome research · 2025Article
- Arabidopsis conditional photosynthesis mutants abc1k1 and var2 accumulate partially processed thylakoid preproteins and are defective in chloroplast biogenesis.Communications biology · 2025Article
- De-etiolation is Almost Color Blind: The Study of Photosynthesis Awakening under Blue and Red Light.Plant & cell physiology · 2024Article
- Membrane protein provision controls prothylakoid biogenesis in tobacco etioplasts.The Plant cell · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light triggers chloroplast differentiation whereby the etioplast transforms into a photosynthesizing chloroplast and the thylakoid rapidly emerges. However, the sequence of events during chloroplast differentiation remains poorly understood. Using Serial Block Face Scanning Electron Microscopy (SBF-SEM), we generated a series of chloroplast 3D reconstructions during differentiation, revealing chloroplast number and volume and the extent of envelope and thylakoid membrane surfaces. Furthermore, we used quantitative lipid and whole proteome data to complement the (ultra)structural data, providing a time-resolved, multi-dimensional description of chloroplast differentiation. This showed two distinct phases of chloroplast biogenesis: an initial photosynthesis-enabling 'Structure Establishment Phase' followed by a 'Chloroplast Proliferation Phase' during cell expansion. Moreover, these data detail thylakoid membrane expansion during de-etiolation at the seedling level and the relative contribution and differential regulation of proteins and lipids at each developmental stage. Altogether, we establish a roadmap for chloroplast differentiation, a critical process for plant photoautotrophic growth and survival.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.