ArticleMolecular plant pathology2021
Identification of viral particles in the apoplast of Nicotiana benthamiana leaves infected by potato virus X.
Article in Molecular plant pathology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review.Current microbiology · 2026Review
- Streamlined molecular farming of plant virus therapeutics for space flight and other low-resource environments.NPJ science of plants · 2026Article
- Viral tropism in plants, reproductive tissues, and seeds.Archives of microbiology · 2025Review
- Identification of mungbean yellow mosaic India virus and susceptibility-related metabolites in the apoplast of mung bean leaves.Plant cell reports · 2024Article
- Plant Extracellular Vesicles: Current Landscape and Future Directions.Plants (Basel, Switzerland) · 2023Review
- A papain-like cysteine protease-released small signal peptide confers wheat resistance to wheat yellow mosaic virus.Nature communications · 2023Article
- dsRNA-induced immunity targets plasmodesmata and is suppressed by viral movement proteins.The Plant cell · 2023Article
- Plant production of a virus-like particle-based vaccine candidate against porcine reproductive and respiratory syndrome.Frontiers in plant science · 2023Article
- Molecular mechanisms underlying host-induced gene silencing.The Plant cell · 2022Review
- Identification of viral particles in the apoplast of Nicotiana benthamiana leaves infected by potato virus X.Molecular plant pathology · 2021Article
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The apoplast is the extracellular space for signalling, nutrient transport, and plant-microbe interactions, but little is known about how plant viruses use the foliar apoplast. Proteomic analysis of the apoplasts isolated from potato virus X (PVX)-infected Nicotiana benthamiana plants showed that the coat protein (CP) is the dominant viral component. The presence of the CP in the apoplast was confirmed by western blot, viral nucleic acid was detected by reverse transcription-PCR and northern blot, and viral particles were observed by transmission electron microscopy (TEM). The apoplast from infected leaves was infectious if rubbed onto healthy leaves but not when infiltrated into them. The exosomes were separated from the apoplast fluid by high-speed centrifugation and TEM showed that PVX particles were not associated with the exosomes. These results suggest that PVX virions are released to the N. benthamiana apoplast in a one-way manner and do not share the bidirectional transport of exosomes.
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