Evidence map›Paper›PMID 33627831›Full record

ArticleCommunications biology2021

βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

Sayan Ghosh, Haitao Liu, Meysam Yazdankhah, Nadezda Stepicheva, Peng Shang, Tanuja Vaidya, Stacey Hose, Urvi Gupta, Michael Joseph Calderon, Ming-Wen Hu and 19 more

Open access · goldAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
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  4. Article
  5. Review
  6. Article
  7. Thermal Shift Assay in Ferroptosis.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  8. Article
  9. Article
  10. Review
  11. Retina regeneration: lessons from vertebrates.Oxford open neuroscience · 2022
    Review
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors at 6 institutions in 3 countries.

Sayan GhoshDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Haitao Liu *Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Meysam Yazdankhah *Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Nadezda Stepicheva *Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Peng Shang *Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Tanuja VaidyaGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, India.
Stacey HoseDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Urvi GuptaDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Michael Joseph CalderonDepartment of Cell Biology and Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Ming-Wen HuWilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Archana Padmanabhan NairGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, India.
Joseph WeissDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Christopher S FittingDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Imran A BhuttoWilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Santosh Gopi Krishna GaddeGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, India.
Naveen Kumar NaikGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, India.
Chaitra JaydevGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, India.ORCID http://orcid.org/0000-0001-8392-7159
Gerard A LuttyWilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
James T HandaWilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ashwath JayagopalKodiak Sciences, Palo Alto, CA, USA.
Jiang QianWilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-0476-3596
José-Alain SahelDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Dhivyaa RajasundaramDepartment of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Yuri SergeevNational Eye Institute, National Institutes of Health, Bethesda, MD, USA.ORCID http://orcid.org/0000-0002-7204-6572
J Samuel ZiglerWilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Swaminathan SethuGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, India.
Simon WatkinsDepartment of Cell Biology and Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.ORCID http://orcid.org/0000-0003-4092-1552
Arkasubhra GhoshGROW Research Laboratory, Narayana Nethralaya Foundation, Bengaluru, India.
Debasish SinhaDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Debasish@pitt.edu.
University of Pittsburgh · USJohns Hopkins University · USNarayana Nethralaya · INCentre National de la Recherche Scientifique · FRKodiak Sciences · USNational Institutes of Health · US

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Ji Yi · 1985 to 2026
$22.1M
Proteins From Hereditary Eye Diseases: In-silico and Experimental StudiesZIAEY000476 · NEI · NATIONAL EYE INSTITUTE · PI SERGEEV, YURI · 2009 to 2025
$16.8M
A novel bA3/A1-crystallin gene mutation results in persistent fetal vasculatureR01EY019037 · NEI · JOHNS HOPKINS UNIVERSITY · PI SINHA, DEBASISH · 2008 to 2015
$4.1M
NEI NIH HHS P30 EY001765NEI NIH HHS R01 EY019037
6 · The paper itself

Abstract

βA3/A1-crystallin, a lens protein that is also expressed in astrocytes, is produced as βA3 and βA1-crystallin isoforms by leaky ribosomal scanning. In a previous human proteome high-throughput array, we found that βA3/A1-crystallin interacts with protein tyrosine phosphatase 1B (PTP1B), a key regulator of glucose metabolism. This prompted us to explore possible roles of βA3/A1-crystallin in metabolism of retinal astrocytes. We found that βA1-crystallin acts as an uncompetitive inhibitor of PTP1B, but βA3-crystallin does not. Loss of βA1-crystallin in astrocytes triggers metabolic abnormalities and inflammation. In CRISPR/cas9 gene-edited βA1-knockdown (KD) mice, but not in βA3-knockout (KO) mice, the streptozotocin (STZ)-induced diabetic retinopathy (DR)-like phenotype is exacerbated. Here, we have identified βA1-crystallin as a regulator of PTP1B; loss of this regulation may be a new mechanism by which astrocytes contribute to DR. Interestingly, proliferative diabetic retinopathy (PDR) patients showed reduced βA1-crystallin and higher levels of PTP1B in the vitreous humor.

Indexed as

Energy MetabolismAnimalsAstrocytesbeta-Crystallin A ChainCase-Control StudiesCells, CulturedCrystallinsDiabetic RetinopathyDisease Models, AnimalGlucoseHumansMiceMice, Inbred C57BLMice, KnockoutMitochondriaProtein BindingbetaA3-crystallin, ratbeta-Crystallin A ChainCRYBA1 protein, humanCryba1 protein, mouseCrystallinsGlucoseProtein Tyrosine Phosphatase, Non-Receptor Type 1PTPN1 protein, humanPtpn1 protein, mousePtpn1 protein, rat

Identifiers

PMID33627831
PMCPMC7904954
OpenAlexW3129248076

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.