Evidence map›Paper›PMID 33627695›Full record

ArticleScientific reports2021

Optogenetic current in myofibroblasts acutely alters electrophysiology and conduction of co-cultured cardiomyocytes.

Geran M Kostecki, Yu Shi, Christopher S Chen, Daniel H Reich, Emilia Entcheva, Leslie Tung

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 23 citations in OpenAlex.

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  12. Cardiac optogenetics: a decade of enlightenment.Nature reviews. Cardiology · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Geran M KosteckiDepartment of Biomedical Engineering, Johns Hopkins University, 720 Rutland Ave., Baltimore, MD, 21205, USA.
Yu ShiDepartment of Physics and Astronomy, Johns Hopkins University, Baltimore, MD, 21218, USA.
Christopher S ChenBiological Design Center, Department of Biomedical Engineering, Boston University, Boston, MA, USA.
Daniel H ReichDepartment of Physics and Astronomy, Johns Hopkins University, Baltimore, MD, 21218, USA.
Emilia EntchevaDepartment of Biomedical Engineering, George Washington University, Washington, DC, USA.
Leslie TungDepartment of Biomedical Engineering, Johns Hopkins University, 720 Rutland Ave., Baltimore, MD, 21205, USA. ltung@jhu.edu.
Johns Hopkins University · USBoston University · USGeorge Washington University · US

Funding

Cardiac Optogenetics: A Cell Delivery ApproachR01HL111649 · NHLBI · STATE UNIVERSITY NEW YORK STONY BROOK · PI ENTCHEVA, EMILIA · 2012 to 2016
$2.4M
Mechanoelectrical Interactions Between Cardiac Myofibroblasts and MyocytesR01HL127087 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI TUNG, LESLIE · 2016 to 2019
$2.0M
Request for Zeiss LSM 510 MP Multiphoton Imaging SystemS10RR024550 · NCRR · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2008 to 2008
$500k
NCRR NIH HHS S10 RR024550NHLBI NIH HHS R01 HL127087NIH HHS R01HL111649
6 · The paper itself

Abstract

Interactions between cardiac myofibroblasts and myocytes may slow conduction and generate spontaneous beating in fibrosis, increasing the chance of life-threatening arrhythmia. While co-culture studies have shown that myofibroblasts can affect cardiomyocyte electrophysiology in vitro, the extent of myofibroblast-myocyte electrical conductance in a syncytium is unknown. In this neonatal rat study, cardiac myofibroblasts were transduced with Channelrhodopsin-2, which allowed acute and selective increase of myofibroblast current, and plated on top of cardiomyocytes. Optical mapping revealed significantly decreased conduction velocity (- 27 ± 6%, p < 10

Indexed as

Action PotentialsAnimalsArrhythmias, CardiacCardiac ElectrophysiologyCells, CulturedCoculture TechniquesElectrophysiological PhenomenaFibrosisHeart RateMyocytes, CardiacMyofibroblastsOptogeneticsRats

Identifiers

PMID33627695
PMCPMC7904933
OpenAlexW3129859597

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.