Evidence map›Paper›PMID 33627162›Full record

ArticleJournal of ovarian research2021

Exosome-mediated transfer of CD44 from high-metastatic ovarian cancer cells promotes migration and invasion of low-metastatic ovarian cancer cells.

Xiameng Shen, Conghui Wang, Huihui Zhu, Yaping Wang, Xinyu Wang, Xiaodong Cheng, Wanzhong Ge, Weiguo Lu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiameng Shen *Department of Gynecologic Oncology, Women's Hospital School of Medicine Zhejiang University, No. 1 Xueshi Road, Hangzhou, 310006, China.
Conghui Wang *Women's Reproductive Health Research Laboratory of Zhejiang Province, Women's Hospital School of Medicine Zhejiang University, Hangzhou, China.
Huihui ZhuDepartment of Gynecologic Oncology, Women's Hospital School of Medicine Zhejiang University, No. 1 Xueshi Road, Hangzhou, 310006, China.
Yaping WangDepartment of Gynecologic Oncology, Women's Hospital School of Medicine Zhejiang University, No. 1 Xueshi Road, Hangzhou, 310006, China.
Xinyu WangDepartment of Gynecologic Oncology, Women's Hospital School of Medicine Zhejiang University, No. 1 Xueshi Road, Hangzhou, 310006, China.
Xiaodong ChengDepartment of Gynecologic Oncology, Women's Hospital School of Medicine Zhejiang University, No. 1 Xueshi Road, Hangzhou, 310006, China.
Wanzhong GeWomen's Reproductive Health Research Laboratory of Zhejiang Province, Women's Hospital School of Medicine Zhejiang University, Hangzhou, China.
Weiguo LuDepartment of Gynecologic Oncology, Women's Hospital School of Medicine Zhejiang University, No. 1 Xueshi Road, Hangzhou, 310006, China. lbwg@zju.edu.cn.ORCID http://orcid.org/0000-0003-2062-7145

Funding

key research and development project of Zhejiang Province 2019C03010Medical and health research project of Zhejiang Province 2017211914National Natural Science Foundation of China 81974402National Natural Science Foundation of China 82072856
6 · The paper itself

Abstract

objectiveTo investigate the detailed roles and mechanisms of tumor-derived exosomes in progression and metastasis of ovarian cancer in vitro.

methodsExosomes were isolated by differential centrifugation method; the morphology, size and biological markers of exosomes were separately defined by transmission electron microscopy, nanoS90 and Western blotting; Trans-well chambers assay was used to assess the ability of migration and invasion of recipient cells uptaking the exosomes from HO8910PM cells. The downstream molecule was screened by mass spectrometry.CD44 was identified by western blotting and the function of CD44 was identified by trans-well chambers assay and CCK8 assay.

resultsExosomes derived from HO8910PM cells could be transferred to HO8910 cells and promote cell migration and invasion in the recipient cells of ovarian cancer. And CD44 could be transferred to the HO8910 cells through exosomes from HO8910PM cells and influence the migration and invasion ability of HO8910 cells.

conclusionThe more aggressive subpopulation can transfer a metastatic phenotype to the less one via secreting exosomes within a heterogeneous tumor. CD44 may be a potential therapeutic approach for ovarian cancer.

Indexed as

Carcinoma, Ovarian EpithelialCell Line, TumorCell MovementExosomesFemaleHumansHyaluronan ReceptorsMass SpectrometryMicroscopy, Electron, TransmissionNeoplasm MetastasisOvarian NeoplasmsPhenotypeHyaluronan ReceptorsExosomeHeterogeneityMetastasisOvarian cancer

Identifiers

PMID33627162
PMCPMC7905574

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.