Evidence map›Paper›PMID 33625672›Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2021

Hypoxia-mediated down-regulation of miRNAs' biogenesis promotes tumor immune escape in bladder cancer.

Y Zhao, Z Shi, Z Hao, J Zhou, C Han, R Li, Q Lv, Y Liu, C Liang

Abstract read
PubMed Publisher
In one paragraph

Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 7 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Y ZhaoDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230000, China.
Z ShiXuzhou Central Hospital, Xuzhou, 221009, China.
Z HaoDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230000, China.
J ZhouDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230000, China.
C HanXuzhou Central Hospital, Xuzhou, 221009, China.
R LiXuzhou Central Hospital, Xuzhou, 221009, China.
Q LvXuzhou Central Hospital, Xuzhou, 221009, China.
Y LiuXuzhou Central Hospital, Xuzhou, 221009, China.
C LiangDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230000, China. chaozhaoliang2020@163.com.ORCID http://orcid.org/0000-0001-6774-8898
Xuzhou Medical College · CNAnhui Medical University · CN

Funding

Jiangsu Medical Innovation Team CXTDA2017048Jiangsu Province, key research and development program BE2019637,BE2017635Jiangsu Province, young medical talents QNRC2016386National Natural Science Foundation of China 81774089Xuzhou Medical Outstanding Talents Xuzhou Health Education Research [2017] No. 22Xuzhou Science and Technology Plan Project KC18036,KC19075
6 · The paper itself

Abstract

backgroundThe study examines the function of hypoxia-mediated down-regulation of microRNAs (miRNAs) (mir-30c, mir-135a, and mir-27a) in the process of bladder cancer immune escape.

methodsQuantitative Real-time PCR (qRT-PCR) was carried out to determine gene expression levels of Drosha and Dicer under hypoxia treatment, while western blotting and flow cytometry were used to determine protein expression. Seven reported miRNAs were identified via qRT-PCR assay. Flow cytometry detection of CD3/CD4/CD8-positive expression and statistics. Enzyme-linked immunosorbent assay (ELISA) detected cellular immune factors content. Cell apoptosis was checked via flow cytometry assay. Luciferase report assay and western blot assays were both used to verify the relationship between miRNAs and Casitas B-lineage lymphoma proto-oncogene b (Cbl-b). The animal model was established and Hematoxylin-eosin (HE) staining, TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, and immunohistochemistry (IHC) assays were separately used to verify the conclusions.

resultsThe CD3 + /CD4 + expression was increased in the hypoxia group, while CD3 + /CD8 + expression, the cellular immune factors content Interleukin-2 (IL-2) and Tumor Necrosis Factor-α (TNFα) along with the cell apoptosis were suppressed. The protein expression of Cbl-b was found to be up-regulated in the hypoxia group. After constructing the overexpression/ knockdown of Cbl-b in peripheral blood mononuclear cell (PBMC), Cbl-b has been found to promote tumor immune escape in bladder cancer. Furthermore, Cbl-b had been identified as the co-targets of mir-30c, mir-135a, and mir-27a and down-regulation of miRNA biogenesis promotes Cbl-b expression and deactivating T cells in vitro/in vivo.

conclusionHypoxia-mediated down-regulation of miRNAs' biogenesis promotes tumor immune escape in bladder cancer, which could bring much more advance to the medical research on tumors.

Indexed as

Adaptor Proteins, Signal TransducingAnimalsApoptosisCD3 ComplexCD4 AntigensCD8 AntigensDEAD-box RNA HelicasesDisease Models, AnimalDown-RegulationGene Expression Regulation, NeoplasticHumansInterleukin-2Leukocytes, MononuclearMaleMiceMice, Inbred C57BLAdaptor Proteins, Signal TransducingCBLB protein, humanCD3 ComplexCD4 AntigensCD8 AntigensDEAD-box RNA HelicasesDICER1 protein, humanDROSHA protein, humanInterleukin-2MAS1 protein, humanMicroRNAsProto-Oncogene MasProto-Oncogene Proteins c-cblRibonuclease IIITumor Necrosis Factor-alphaBladder cancerCbl-bHypoxiaImmune escapemiRNA

Identifiers

PMID33625672
OpenAlexW3133469754

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.