Evidence map›Paper›PMID 33625598›Full record

ArticleMolecular genetics and genomics : MGG2021

Universal fluorescence in situ hybridization (FISH) protocol for mapping repetitive DNAs in insects and other arthropods.

Diogo Cavalcanti Cabral-de-Mello, František Marec

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Molecular genetics and genomics : MGG, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
8.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 55 citations in OpenAlex.

  1. Article
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  3. Satellite DNA evolution in two holocentric species of Edessa true bugs (Hemiptera: Pentatomidae) with unusually high heterochromatin abundance.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026
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  16. The burst of satellite DNA in Leptidea wood white butterflies and their putative role in karyotype evolution.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024
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  17. Satellitome Analysis ofInternational journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Diogo Cavalcanti Cabral-de-MelloDepartamento de Biologia Geral e Aplicada, Instituto de Biociências, UNESP- Universidade Estadual Paulista, Rio Claro, São Paulo, CEP 13506-900, Brazil. cabral.mello@unesp.br.ORCID http://orcid.org/0000-0002-4721-2655
František MarecBiology Centre of the Czech Academy of Sciences, Institute of Entomology, České Budějovice, Czech Republic.ORCID http://orcid.org/0000-0002-6745-5603
Czech Academy of Sciences · CZInstitute of Entomology · CZ

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/097319 and 2019/19069-7Grantová Agentura České Republiky 17-13713S and 20-13784S
6 · The paper itself

Abstract

Repetitive DNAs comprise large portion of eukaryote genomes. In genome projects, the assembly of repetitive DNAs is challenging due to the similarity between repeats, which generate ambiguities for alignment. Fluorescence in situ hybridization (FISH) is a powerful technique for the physical mapping of various sequences on chromosomes. This technique is thus very helpful in chromosome-based genome assemblies, providing information on the fine architecture of genomes and their evolution. However, various protocols are currently used for FISH mapping, most of which are relatively laborious and expensive, or work properly only with a specific type of probes or sequences, and there is a need for a universal and affordable FISH protocol. Here we tested a FISH protocol for mapping of different DNA repeats, such as multigene families (rDNAs, U snDNAs, histone genes), satellite DNAs, microsatellites, transposable elements, DOP-PCR products, and telomeric motif (TTAGG)

Indexed as

AnimalsArthropodsChromosome MappingDNAEvolution, MolecularFluorescenceInsectaIn Situ Hybridization, FluorescenceMultigene FamilyRepetitive Sequences, Nucleic AcidTelomereDNAArthropodaChromosomesCytogeneticsDNA repeatsFISH protocolGenome structureInsecta

Identifiers

PMID33625598
OpenAlexW3132286630

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.