ArticleMicrobial biotechnology2021
Minicell-based fungal RNAi delivery for sustainable crop protection.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- A systematic review of nanocarriers used in medicine and beyond - definition and categorization framework.Journal of nanobiotechnology · 2025Pooled it
- Interactional Endocytosis and Transmembrane Transport Promote Cellular Internalization of Nano-Delivered RNA Drugs for Efficient Control of Crop Diseases.Plant biotechnology journal · 2026Article
- The unfulfilled potential of nanocarriers for RNA delivery in antiviral crop protection.Nature plants · 2026Review
- Control Strategies of Plant Viruses Using Spray-Induced Gene Silencing.The plant pathology journal · 2026Review
- RNA Interference in Plant Interactions with Pathogenic Microorganisms: A Weapon or a Liability?Current issues in molecular biology · 2025Review
- DNA Aptamers Targeting BcSOD1: A Novel Strategy for Controlling Botrytis cinerea in Sustainable Agriculture.Plant biotechnology journal · 2025Article
- RNAi Strategies Against Downy Mildews: Insights Into dsRNA Uptake and Silencing.Molecular plant pathology · 2025Article
- Toxicological and Functional Assessment of Minicell-Encapsulated dsRNA on Biocontrol Agents in Agriculture.ACS environmental Au · 2025Article
- pSIG plasmids, MoClo-compatible vectors for efficient production of chimeric double-stranded RNAs in Escherichia coli HT115 (DE3) strain.Plant methods · 2025Article
- RNAi-Based Approaches to Control Mycotoxin Producers: Challenges and Perspectives.Journal of fungi (Basel, Switzerland) · 2024Review
- Cross-Kingdom RNA Transport Based on Extracellular Vesicles Provides Innovative Tools for Plant Protection.Plants (Basel, Switzerland) · 2024Review
- RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry.Plant cell reports · 2024Article
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Exogenous Application of dsRNA in Plant Protection: Efficiency, Safety Concerns and Risk Assessment.International journal of molecular sciences · 2024Review
- Review
- Advances in bacteria-based drug delivery systems for anti-tumor therapy.Clinical & translational immunology · 2024Review
- Leveraging RNA interference technology for selective and sustainable crop protection.Frontiers in plant science · 2024Review
- Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.Current opinion in plant biology · 2023Review
- Why Do We Need Alternative Methods for Fungal Disease Management in Plants?Plants (Basel, Switzerland) · 2023Review
- Outlook on RNAi-Based Strategies for ControllingPathogens (Basel, Switzerland) · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Spray-induced gene silencing (SIGS) using topical dsRNA applications has risen as a promising, target-specific, and environmentally friendly disease management strategy against phytopathogenic fungi. However, dsRNA stability, efficacy, and scalability are still the main constraints facing SIGS broader application. Here we show that Escherichia coli-derived anucleated minicells can be utilized as a cost-effective, scalable platform for dsRNA production and encapsulation. We demonstrated that minicell-encapsulated dsRNA (ME-dsRNA) was shielded from RNase degradation and stabilized on strawberry surfaces, allowing dsRNA persistence in field-like conditions. ME-dsRNAs targeting chitin synthase class III (Chs3a, Chs3b) and DICER-like proteins (DCL1 and DCL2) genes of Botryotinia fuckeliana selectively knocked-down the target genes and led to significant fungal growth inhibition in vitro. We also observed a compensatory relationship between DCL1 and DCL2 gene transcripts, where the silencing of one gene upregulated the expression of the other. Contrary to naked-dsRNAs, ME-dsRNAs halted disease progression in strawberries for 12 days under greenhouse conditions. These results elucidate the potential of ME-dsRNAs to enable the commercial application of RNAi-based, species-specific biocontrols comparable in efficacy to conventional synthetics. ME-dsRNAs offer a platform that can readily be translated to large-scale production and deployed in open-field applications to control grey mould in strawberries.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.