ArticleThe Plant cell2021
Resolving diurnal dynamics of the chloroplastic glutathione redox state in Arabidopsis reveals its photosynthetically derived oxidation.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Sulfur metabolism-dependent retrograde signaling for oxidative stress acclimation.Journal of experimental botany · 2026Review
- Emerging roles of glutaredoxins in plant metabolism.Journal of experimental botany · 2026Review
- Exogenous N-Acetyl-L-cysteine Improves Soybean Saline-Alkali Tolerance by Enhancing the hGSH Pathway to Alleviate Oxidative Damage.Plants (Basel, Switzerland) · 2026Article
- Molecular Mechanisms and Experimental Strategies for Understanding Plant Drought Response.Plants (Basel, Switzerland) · 2026Review
- Dynamic regulation of Arabidopsis β-AMYLASE1 by glutathione and thioredoxins affects starch in guard cells.Plant physiology · 2025Article
- Nitrogen Assimilation Plays a Role in Balancing the Chloroplastic Glutathione Redox Potential Under High Light Conditions.Plant, cell & environment · 2025Article
- Reactivation and long-term stabilization of the [NiFe] Hox hydrogenase of Synechocystis sp. PCC6803 by glutathione after oxygen exposure.The Journal of biological chemistry · 2025Article
- In planta imaging of pyridine nucleotides using second-generation fluorescent protein biosensors.The Plant journal : for cell and molecular biology · 2024Article
- Chloroplasts lacking class I glutaredoxins are functional but show a delayed recovery of protein cysteinyl redox state after oxidative challenge.Redox biology · 2024Article
- Noninvasive In Planta Live Measurements of HMethods in molecular biology (Clifton, N.J.) · 2024Article
- Lighting the light reactions of photosynthesis by means of redox-responsive genetically encoded biosensors for photosynthetic intermediates.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Review
- NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems.Nature communications · 2023Article
- Suppression of theLife (Basel, Switzerland) · 2023Article
- Reactive oxygen species signalling in plant stress responses.Nature reviews. Molecular cell biology · 2022Review
- ROS production and signalling in chloroplasts: cornerstones and evolving concepts.The Plant journal : for cell and molecular biology · 2022Review
- Systematic monitoring of 2-Cys peroxiredoxin-derived redox signals unveiled its role in attenuating carbon assimilation rate.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress.International journal of molecular sciences · 2022Review
- Sensing stress responses in potato with whole-plant redox imaging.Plant physiology · 2021Article
- A chloroplast-targeted sensor for continuous monitoring of redox status in planta.The Plant cell · 2021Article
- Live monitoring of plant redox and energy physiology with genetically encoded biosensors.Plant physiology · 2021Review
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants are subjected to fluctuations in light intensity, and this might cause unbalanced photosynthetic electron fluxes and overproduction of reactive oxygen species (ROS). Electrons needed for ROS detoxification are drawn, at least partially, from the cellular glutathione (GSH) pool via the ascorbate-glutathione cycle. Here, we explore the dynamics of the chloroplastic glutathione redox potential (chl-EGSH) using high-temporal-resolution monitoring of Arabidopsis (Arabidopsis thaliana) lines expressing the reduction-oxidation sensitive green fluorescent protein 2 (roGFP2) in chloroplasts. This was carried out over several days under dynamic environmental conditions and in correlation with PSII operating efficiency. Peaks in chl-EGSH oxidation during dark-to-light and light-to-dark transitions were observed. Increasing light intensities triggered a binary oxidation response, with a threshold around the light saturating point, suggesting two regulated oxidative states of the chl-EGSH. These patterns were not affected in npq1 plants, which are impaired in non-photochemical quenching. Oscillations between the two oxidation states were observed under fluctuating light in WT and npq1 plants, but not in pgr5 plants, suggesting a role for PSI photoinhibition in regulating the chl-EGSH dynamics. Remarkably, pgr5 plants showed an increase in chl-EGSH oxidation during the nights following light stresses, linking daytime photoinhibition and nighttime GSH metabolism. This work provides a systematic view of the dynamics of the in vivo chloroplastic glutathione redox state during varying light conditions.
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