Evidence map›Paper›PMID 33624396›Full record

ArticleNeurogastroenterology and motility2022

Evidence for the presence and release of BDNF in the neuronal and non-neuronal structures of the internal anal sphincter.

Arjun Singh, Jagmohan Singh, Satish Rattan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurogastroenterology and motility, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
13.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.

  1. Pooled it
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  18. RNA m6A methylation regulators in ovarian cancer.Cancer cell international · 2021
    Review
  19. BDNF rescues aging-associated internal anal sphincter dysfunction.American journal of physiology. Gastrointestinal and liver physiology · 2021
    Article
  20. New Insights Into Cancer Chronotherapies.Frontiers in pharmacology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Arjun SinghDepartment of Medicine, Division of Gastroenterology & Hepatology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.ORCID 0000-0001-6389-5540
Jagmohan SinghDepartment of Pharmacology and Experimental Therapeutics, Jefferson Alumni Hall, Thomas Jefferson University, Philadelphia, PA, USA.
Satish RattanDepartment of Medicine, Division of Gastroenterology & Hepatology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.ORCID 0000-0001-6731-6980
Thomas Jefferson University · US

Funding

NEUROHUMORAL CONTROL OF INTERNAL ANAL SPINCHTERR01DK035385 · NIDDK · THOMAS JEFFERSON UNIVERSITY · PI RATTAN, SATISH · 1986 to 2020
$7.8M
NIDDK NIH HHS R01 DK035385
6 · The paper itself

Abstract

backgroundData on the neuromodulatory effects of brain-derived neurotrophic factor (BDNF) in the gastrointestinal tract were recently reported, but there are still no data on the presence, distribution, and release of BDNF in the gastrointestinal tract, including the internal anal sphincter (IAS).

methodsWe examined the presence and distribution of BDNF and its receptor TrkB in the different IAS structures (neuronal and non-neuronal) via immunohistochemical and immunocytochemical analyses. We also monitored the release of BDNF in an IAS muscle bath (consisting of smooth muscle cells [SMCs], myenteric plexus, and submucosal plexus) before and after different agonists, and electrical field stimulation in the absence and presence of neurotoxin tetrodotoxin. KEY

resultsBDNF/TrkB was found to be present in all layers of the IAS, especially the smooth muscle, mucosa, myenteric plexus, and submucosal plexus. Detailed analyses revealed a significant colocalization between BDNF and TrkB in different structures, especially in the smooth muscle, the SMCs, and both plexuses. Data further showed higher levels of BDNF in the cytosol and that of TrkB toward the periphery of the SMCs. CONCLUSIONS & INFERENCES: These studies showed that BDNF/TrkB was present not only in the enteric nervous system (ENS), but also in the SMCs. For the neuromodulatory effects, BDNF is released locally from the ENS ((myenteric (10.01 ± 0.23 pg/ml) and submucosal plexus (9.05 ± 0.51 pg/ml)) and the SMCs (18.63 ± 1.63 pg/ml). Collectively, these findings have pathophysiological and therapeutic implications regarding the role of BDNF/TrkB in the IAS-associated rectoanal motility disorders.

Indexed as

Brain-Derived Neurotrophic FactorEnteric Nervous SystemAnal CanalMuscle, SmoothNeuronsBrain-Derived Neurotrophic FactorBDNFenteric nervous systemneurotrophic factorsmooth muscle toneTrkB signaling

Identifiers

PMID33624396
PMCPMC9558559
OpenAlexW3129721376

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.