Evidence map›Paper›PMID 33623093›Full record

ArticleScientific reports2021

High diversity droplet microfluidic libraries generated with a commercial liquid spotter.

Jesse Q Zhang, Christian A Siltanen, Ata Dolatmoradi, Chen Sun, Kai-Chun Chang, Russell H Cole, Zev J Gartner, Adam R Abate

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Droplet-Based Microfluidics: Applications in Pharmaceuticals.Pharmaceuticals (Basel, Switzerland) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jesse Q ZhangDepartment of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Christian A Siltanen *Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Ata Dolatmoradi *Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Chen SunDepartment of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Kai-Chun ChangDepartment of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.
Russell H ColeScribe Biosciences, Inc., San Francisco, CA, USA.
Zev J GartnerUC Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco, San Francisco, CA, USA.
Adam R AbateDepartment of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA. adam@abatelab.org.

Funding

NIH HHS 1R43HG010128-01
6 · The paper itself

Abstract

Droplet libraries consisting of many reagents encapsulated in separate droplets are necessary for applications of microfluidics, including combinatorial chemical synthesis, DNA-encoded libraries, and massively multiplexed PCR. However, existing approaches for generating them are laborious and impractical. Here, we describe an automated approach using a commercial array spotter. The approach can controllably emulsify hundreds of different reagents in a fraction of the time of manual operation of a microfluidic device, and without any user intervention. We demonstrate that the droplets produced by the spotter are similarly uniform to those produced by microfluidics and automate the generation of a ~ 2 mL emulsion containing 192 different reagents in ~ 4 h. The ease with which it can generate high diversity droplet libraries should make combinatorial applications more feasible in droplet microfluidics. Moreover, the instrument serves as an automated droplet generator, allowing execution of droplet reactions without microfluidic expertise.

Indexed as

Automation, LaboratoryEmulsionsHigh-Throughput Screening AssaysLipidsMicrofluidicsSmall Molecule LibrariesEmulsionsLipidsSmall Molecule Libraries

Identifiers

PMID33623093
PMCPMC7902812

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.